Lutsch C, Cesbron J Y, Henry D, Dessaint J P, Wandji K, Ismail M, Capron A
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM 167-CNRS 624, Institut Pasteur, Lille, France.
Clin Exp Immunol. 1988 Feb;71(2):253-60.
A two-site immunoradiometric assay using a monoclonal antibody (MoAb) against Brugia malayi microfilariae allowed the detection of parasite molecules both in the serum and the urine of patients from Sri Lanka infected with Wuchereria bancrofti. Whereas 50% of patients had no antigen in their serum, all of them excreted detectable amounts of antigen in their urine, the levels being higher in symptomatic than in asymptomatic patients. The poor detection in serum appeared to be related to the presence of circulating immune complexes. It was shown that the isotype of the antibodies complexed with the circulating antigen was IgM in the asymptomatic group, while it was mainly IgG in the symptomatic patients (swelling and lymphoedema or elephantiasis). These results suggest the existence of regulatory immune mechanisms affecting the clinical expression of lymphatic filariasis.
一种使用抗马来布鲁线虫微丝蚴单克隆抗体（MoAb）的双位点免疫放射分析方法，能够检测来自斯里兰卡感染班氏吴策线虫患者的血清和尿液中的寄生虫分子。虽然50%的患者血清中没有抗原，但所有患者尿液中均排泄出可检测量的抗原，有症状患者的抗原水平高于无症状患者。血清中检测不佳似乎与循环免疫复合物的存在有关。结果表明，无症状组中与循环抗原结合的抗体的同种型为IgM，而有症状患者（肿胀、淋巴水肿或象皮肿）中主要为IgG。这些结果提示存在影响淋巴丝虫病临床表型的调节性免疫机制。
