圆锥角膜上皮改变的组织学模式
Histological Patterns of Epithelial Alterations in Keratoconus.
作者信息
Crespo Millas Sara, López José Carlos, García-Lagarto Elena, Obregón Estibaliz, Hileeto Denise, Maldonado Miguel J, Pastor J Carlos
机构信息
Hospital Clínico Universitario de Valladolid (HCUV), Valladolid, Spain.
Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Valladolid, Spain.
出版信息
J Ophthalmol. 2020 Jul 30;2020:1468258. doi: 10.1155/2020/1468258. eCollection 2020.
PURPOSE
The purpose of this study was to confirm the presence of specific patterns of epithelial response in corneal buttons from keratoconus patients.
METHODS
This was a retrospective and descriptive study. 90 penetrating keratoplasty specimens obtained from patients diagnosed with keratoconus were evaluated using bright-field microscopy. Morphologically identifiable characteristics including epithelial cell density and epithelial thickness were analyzed on hematoxylin and eosin- (H&E-) and periodic acid of Schiff- (PAS-) stained slides.
RESULTS
Three distinctive patterns of epithelial alteration of the central cornea were established. Pattern 3, in which the central epithelium was as thick as peripheral epithelium, was the commonest (44.4%), followed by the pattern 2, defined as central epithelium thinner than periphery epithelium (38.9%), and the uncommonest pattern was number 1, with central epithelium thicker than the periphery (16.7%).
CONCLUSIONS
Three distinctive histologic patterns that could potentially have a diagnostic and prognostic value in keratoconus patients were found.
目的
本研究的目的是证实圆锥角膜患者角膜植片上皮反应的特定模式的存在。
方法
这是一项回顾性描述性研究。使用明视野显微镜对90例诊断为圆锥角膜患者的穿透性角膜移植标本进行评估。在苏木精和伊红（H&E）染色及过碘酸希夫（PAS）染色的载玻片上分析形态学上可识别的特征，包括上皮细胞密度和上皮厚度。
结果
确定了中央角膜上皮改变的三种不同模式。模式3（中央上皮与周边上皮一样厚）最为常见（44.4%），其次是模式2（定义为中央上皮比周边上皮薄）（38.9%），最不常见的是模式1（中央上皮比周边厚）（16.7%）。
结论
发现了三种不同的组织学模式，它们可能对圆锥角膜患者具有诊断和预后价值。
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