菌株Yrt2a应激调节基因和毒力基因作为对酸应激的反应的表达
Expression of stress regulator and virulence genes of strain Yrt2a as a response to acid stress.
作者信息
Maerani Maerani, Dewanti-Hariyadi Ratih, Nurjanah Siti
机构信息
Department of Food Science and Technology, Bogor Agricultural University, Bogor, Kampus IPB Dramaga, PO Box 220 16002, West Java 16680 Indonesia.
Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Bogor Agricultural University, Bogor, West Java 16680 Indonesia.
出版信息
Food Sci Biotechnol. 2020 May 3;29(9):1273-1279. doi: 10.1007/s10068-020-00763-1. eCollection 2020 Sep.
Abstract
This research aimed to evaluate the effect of acid stress on the expression of stress regulator ( and ) and virulence ( and ) genes of Yrt2a. The results showed that Yrt2a experienced decrease in number during acid stress and was no longer culturable 90 min post exposure to pH 3.0. During acid stress, the expression of , , and was upregulated by 2.15; 2.19; 1.55; 1.1 and 1.41 log, respectively. However, all genes expression was downregulated when the bacteria entered the unculturable state. The expression of gene decreased to 1.04; 0.37; 0.84 and 1.71 log, respectively; while gene expression reached a level lower than that of control. This research implies a supposition that during acid stress, was capable of maintaining its culturability and pathogenicity until they are no longer culturable.
摘要
本研究旨在评估酸应激对Yrt2a应激调节基因（和）及毒力基因（和）表达的影响。结果显示，Yrt2a在酸应激期间数量减少，暴露于pH 3.0 90分钟后不再可培养。在酸应激期间，、、和的表达分别上调了2.15、2.19、1.55、1.1和1.41个对数。然而，当细菌进入不可培养状态时，所有基因的表达均下调。基因的表达分别降至1.04、0.37、0.84和1.71个对数；而基因表达达到低于对照的水平。本研究表明一种假设，即在酸应激期间，能够维持其可培养性和致病性，直到它们不再可培养。
相似文献
1
Expression of stress regulator and virulence genes of strain Yrt2a as a response to acid stress.菌株Yrt2a应激调节基因和毒力基因作为对酸应激的反应的表达
Food Sci Biotechnol. 2020 May 3;29(9):1273-1279. doi: 10.1007/s10068-020-00763-1. eCollection 2020 Sep.
2
Expression of S, A and genes of strain Yrt2a during stress and viable but nonculturable state.应激及活的非可培养状态下Yrt2a菌株的S、A和基因表达
Food Sci Biotechnol. 2018 Jan 18;27(3):915-920. doi: 10.1007/s10068-018-0313-5. eCollection 2018 Jun.
3
The Glutaredoxin Gene, , Affects Acid Tolerance, Surface Hydrophobicity, Auto-Aggregation, and Biofilm Formation in .谷氧还蛋白基因 影响 中的酸耐受性、表面疏水性、自聚集和生物膜形成。 （你提供的原文中“谷氧还蛋白基因”和“影响”之间似乎有缺失内容）
Front Microbiol. 2018 Feb 5;9:133. doi: 10.3389/fmicb.2018.00133. eCollection 2018.
4
Occurrence of virulence factors in Cronobacter sakazakii and Cronobacter malonaticus originated from clinical samples.源于临床样本的阪崎克罗诺杆菌和丙二酸克罗诺杆菌中毒力因子的出现情况。
Microb Pathog. 2019 Feb;127:250-256. doi: 10.1016/j.micpath.2018.12.011. Epub 2018 Dec 11.
5
Hfq plays important roles in virulence and stress adaptation in Cronobacter sakazakii ATCC 29544.Hfq在阪崎肠杆菌ATCC 29544的毒力和应激适应中发挥重要作用。
Infect Immun. 2015 May;83(5):2089-98. doi: 10.1128/IAI.03161-14. Epub 2015 Mar 9.
6
The role of the general stress response regulator RpoS in Cronobacter sakazakii biofilm formation.一般应激反应调节因子RpoS在阪崎肠杆菌生物膜形成中的作用。
Food Res Int. 2020 Oct;136:109508. doi: 10.1016/j.foodres.2020.109508. Epub 2020 Jul 8.
7
The Role of in Stress Adaptation and Virulence in BAA-894.在BAA - 894中 在应激适应和毒力方面的作用
Foods. 2022 Sep 2;11(17):2680. doi: 10.3390/foods11172680.
8
A Negative Regulator of Cellulose Biosynthesis, , Affects Biofilm Formation, and Adhesion/Invasion Ability of .纤维素生物合成的负调控因子 影响 的生物膜形成及黏附/侵袭能力。
Front Microbiol. 2017 Sep 26;8:1839. doi: 10.3389/fmicb.2017.01839. eCollection 2017.
9
Stress tolerant virulent strains of Cronobacter sakazakii from food.食品中耐应激的阪崎克罗诺杆菌强毒株。
Biol Res. 2014 Nov 25;47(1):63. doi: 10.1186/0717-6287-47-63.
10
Profiling of Virulence Determinants in Cronobacter sakazakii Isolates from Different Plant and Environmental Commodities.来自不同植物和环境商品的阪崎肠杆菌分离株毒力决定因素分析
Curr Microbiol. 2017 May;74(5):560-565. doi: 10.1007/s00284-017-1219-9. Epub 2017 Mar 3.
引用本文的文献
1
Probiotic Saccharomyces boulardii Against Cronobacter sakazakii Infection: In Vitro and In Vivo Studies.益生菌布拉氏酵母菌抗阪崎肠杆菌感染的体外和体内研究
Probiotics Antimicrob Proteins. 2025 Mar 21. doi: 10.1007/s12602-025-10524-3.
2
γ-Aminobutyric Acid (GABA) Metabolic Bypass Plays a Crucial Role in Stress Tolerance and Biofilm Formation in ATCC 29544.γ-氨基丁酸（GABA）代谢旁路在ATCC 29544的胁迫耐受性和生物膜形成中起关键作用。
Foods. 2025 Jan 8;14(2):171. doi: 10.3390/foods14020171.
3
RecA deletion disrupts protein homeostasis, leading to deamidation, oxidation, and impaired glycolysis in .RecA基因缺失会破坏蛋白质稳态，导致脱酰胺、氧化以及糖酵解受损。
Appl Environ Microbiol. 2025 Jan 31;91(1):e0197124. doi: 10.1128/aem.01971-24. Epub 2024 Dec 31.
4
VBNC Cronobacter sakazakii survives in macrophages by resisting oxidative stress and evading recognition by macrophages.阪崎克罗诺杆菌 VBNC 能通过抵抗氧化应激和逃避巨噬细胞识别而在巨噬细胞内存活。
BMC Microbiol. 2024 Nov 6;24(1):458. doi: 10.1186/s12866-024-03595-9.
5
The viable but non-culturable (VBNC) status of Shewanella putrefaciens (S. putrefaciens) with thermosonication (TS) treatment.热声处理下腐败希瓦氏菌（Shewanella putrefaciens，S. putrefaciens）的存活但非可培养状态（VBNC）。
Ultrason Sonochem. 2024 Oct;109:107008. doi: 10.1016/j.ultsonch.2024.107008. Epub 2024 Jul 30.
6
Comprehensive Genomic Characterization of Isolates from Infant Formula Processing Facilities Using Whole-Genome Sequencing.使用全基因组测序对婴儿配方奶粉加工设施分离株进行综合基因组特征分析。
Microorganisms. 2023 Nov 11;11(11):2749. doi: 10.3390/microorganisms11112749.
7
DnaK-Mediated Protein Deamidation: a Potential Mechanism for Virulence and Stress Adaptation in Cronobacter sakazakii.DnaK 介导的蛋白脱酰胺作用：阪崎克罗诺杆菌毒力和应激适应的潜在机制。
Appl Environ Microbiol. 2023 Jul 26;89(7):e0050523. doi: 10.1128/aem.00505-23. Epub 2023 Jun 29.
8
Bacterial Stress Responses as Potential Targets in Overcoming Antibiotic Resistance.细菌应激反应作为克服抗生素耐药性的潜在靶点
Microorganisms. 2022 Jul 9;10(7):1385. doi: 10.3390/microorganisms10071385.
9
Current and Future Perspectives on the Role of Probiotics, Prebiotics, and Synbiotics in Controlling Pathogenic Spp. in Infants.益生菌、益生元及合生元在控制婴儿致病菌种方面作用的当前及未来展望
Front Microbiol. 2021 Oct 21;12:755083. doi: 10.3389/fmicb.2021.755083. eCollection 2021.
本文引用的文献
1
Expression of S, A and genes of strain Yrt2a during stress and viable but nonculturable state.应激及活的非可培养状态下Yrt2a菌株的S、A和基因表达
Food Sci Biotechnol. 2018 Jan 18;27(3):915-920. doi: 10.1007/s10068-018-0313-5. eCollection 2018 Jun.
2
The Glutaredoxin Gene, , Affects Acid Tolerance, Surface Hydrophobicity, Auto-Aggregation, and Biofilm Formation in .谷氧还蛋白基因 影响 中的酸耐受性、表面疏水性、自聚集和生物膜形成。 （你提供的原文中“谷氧还蛋白基因”和“影响”之间似乎有缺失内容）
Front Microbiol. 2018 Feb 5;9:133. doi: 10.3389/fmicb.2018.00133. eCollection 2018.
3
Pathogens of Food Animals: Sources, Characteristics, Human Risk, and Methods of Detection.食用动物的病原体：来源、特征、对人类的风险及检测方法
Adv Food Nutr Res. 2017;82:277-365. doi: 10.1016/bs.afnr.2016.12.009. Epub 2017 Feb 7.
4
hfq regulates acid tolerance and virulence by responding to acid stress in Shigella flexneri.hfq通过对福氏志贺菌的酸应激作出反应来调节酸耐受性和毒力。
Res Microbiol. 2015 Jul-Aug;166(6):476-85. doi: 10.1016/j.resmic.2015.06.007. Epub 2015 Jun 24.
5
Hfq plays important roles in virulence and stress adaptation in Cronobacter sakazakii ATCC 29544.Hfq在阪崎肠杆菌ATCC 29544的毒力和应激适应中发挥重要作用。
Infect Immun. 2015 May;83(5):2089-98. doi: 10.1128/IAI.03161-14. Epub 2015 Mar 9.
6
Coping with low pH: molecular strategies in neutralophilic bacteria.应对低 pH 值：中性菌中的分子策略。
FEMS Microbiol Rev. 2014 Nov;38(6):1091-125. doi: 10.1111/1574-6976.12076. Epub 2014 Jul 2.
7
Polymorphisms in rpoS and stress tolerance heterogeneity in natural isolates of Cronobacter sakazakii.rpoS 多态性与阪崎克罗诺杆菌天然分离株应激耐受性异质性。
Appl Environ Microbiol. 2012 Jun;78(11):3975-84. doi: 10.1128/AEM.07835-11. Epub 2012 Mar 23.
8
General stress sigma factor RpoS influences time required to enter the viable but non-culturable state in Salmonella enterica.普遍应激σ因子 RpoS 影响沙门氏菌进入可存活但非可培养状态所需的时间。
Microbiol Immunol. 2012 Apr;56(4):228-37. doi: 10.1111/j.1348-0421.2012.00428.x.
9
Recruitment of dendritic cells is responsible for intestinal epithelial damage in the pathogenesis of necrotizing enterocolitis by Cronobacter sakazakii.阪崎克罗诺杆菌通过招募树突状细胞导致坏死性小肠结肠炎发病过程中的肠道上皮损伤。
J Immunol. 2011 Jun 15;186(12):7067-79. doi: 10.4049/jimmunol.1100108. Epub 2011 May 6.
10
Impact of environmental stress desiccation, acidity, alkalinity, heat or cold on antibiotic susceptibility of Cronobacter sakazakii.环境胁迫干燥、酸、碱、热或冷对阪崎克罗诺杆菌抗生素敏感性的影响。
Int J Food Microbiol. 2011 Mar 30;146(2):137-43. doi: 10.1016/j.ijfoodmicro.2011.02.013. Epub 2011 Feb 26.
Zotero 插件