Singh Niharika, Raghav Mamta, Narula Shifa, Tandon Simran, Goel Gunjan
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, 173234, India.
Amity Institute of Biotechnology, Amity University, Noida, 201301, India.
Curr Microbiol. 2017 May;74(5):560-565. doi: 10.1007/s00284-017-1219-9. Epub 2017 Mar 3.
Cronobacter sakazakii is an emerging pathogen causing meningitis, sepsis and necrotizing enterocolitis in neonates and immune-compromised adults. The present study describes the profiling of different virulence factors associated with C. sakazakii isolates derived from plant-based materials and environmental samples (soil, water, and vacuum dust). All the isolates exhibited β-hemolysis and chitinase activity, and were able to utilize inositol. Among the nine virulence-associated genes, hly gene coding for hemolysin was detected in all the isolates followed by ompA (outer membrane protein); however, plasmid-borne genes were detected at a level of 60% for both cpa (cronobacter plasminogen activator) and eitA (Ferric ion transporter protein) gene, respectively. Furthermore, the isolate C. sakazakii N81 showed cytotoxicity for Caco-2 cells. The presence of the virulence determinants investigated in this study indicates the pathogenic potential of C. sakazakii with their plausible connection with clinical manifestations.
阪崎肠杆菌是一种新出现的病原体，可导致新生儿和免疫功能低下的成年人发生脑膜炎、败血症和坏死性小肠结肠炎。本研究描述了从植物性材料和环境样本（土壤、水和真空灰尘）中分离得到的阪崎肠杆菌不同毒力因子的特征。所有分离株均表现出β-溶血和几丁质酶活性，并且能够利用肌醇。在九个与毒力相关的基因中，所有分离株均检测到编码溶血素的hly基因，其次是ompA（外膜蛋白）；然而，质粒携带的cpa（阪崎肠杆菌纤溶酶原激活剂）基因和eitA（铁离子转运蛋白）基因的检出水平分别为60%。此外，阪崎肠杆菌N81分离株对Caco-2细胞具有细胞毒性。本研究中所调查的毒力决定因素的存在表明阪崎肠杆菌具有致病潜力，且与临床表现可能存在关联。
