淫羊藿苷通过抑制细胞因子诱导的中性粒细胞趋化因子-1 的分泌缓解大鼠腰痛。

Icariin Ameliorates Lower Back Pain in Rats via Suppressing the Secretion of Cytokine-Induced Neutrophil Chemoatractant-1.

机构信息

Henan Luoyang Orthopedic Hospital (Henan Provincial Orthopedic Hospital), Zhengzhou 450000, China.

Henan Provincial Orthopedic Institute, Zhengzhou 450000, China.

出版信息

Biomed Res Int. 2020 Aug 5;2020:4670604. doi: 10.1155/2020/4670604. eCollection 2020.

PURPOSE

To investigate whether icariin (ICA), a well-known medicine extracted from the stem and leaf of Maxim, had analgesic effect on lower back pain (LBP) in rats.

METHODS

In a puncture-induced LBP rat model, the severity of LBP was quantified using the paw/foot withdrawal threshold method after intragastric administration of ICA at a dosage of 50 mg/kg/d or 100 mg/kg/d. The pain-related peptides of substance P (SP) and calcitonin gene-related peptide (CGRP) were also measured in intervertebral disc (IVD) tissue using RT-PCR after ICA treatment. In addition, the expression of cytokine-induced neutrophil chemoattractant-1 (CINC-1) in IVD was quantified using RT-PCR and ELISA examination.

RESULTS

ICA treatment resulted in a significant amelioration of mechanical allodynia in a dose-response manner, and the analgesic effect could last for two weeks even during the washout period. More importantly, the mechanism of analgesic pharmacological effect in ICA was to suppress the upregulated CINC-1, the homolog of IL-8 in rats, which is a crucial proalgesic factor contributing to LBP, in IVDs.

CONCLUSION

ICA is a novel herbal extract to relieve LBP, and it may be a promising alternative pain killer in the future.

目的

研究从淫羊藿茎叶中提取的著名药物淫羊藿苷（ICA）对大鼠腰痛（LBP）是否具有镇痛作用。

方法

在穿刺诱导的 LBP 大鼠模型中，通过灌胃给予 ICA 剂量为 50mg/kg/d 或 100mg/kg/d，用足底/足退缩阈值法来量化 LBP 的严重程度。在用 ICA 处理后，还通过 RT-PCR 测量椎间盘（IVD）组织中与疼痛相关的肽物质 P（SP）和降钙素基因相关肽（CGRP）的水平。此外，通过 RT-PCR 和 ELISA 检测来定量 IVD 中细胞因子诱导的中性粒细胞趋化因子-1（CINC-1）的表达。