Vega-Dominguez Perla, Peterson Eliza, Pan Min, Di Maio Alessandro, Singh Saumya, Umapathy Siva, Saini Deepak K, Baliga Nitin, Bhatt Apoorva
School of Biosciences and Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
Institute for Systems Biology, Seattle, WA 98109, USA.
Cell Surf. 2020 Aug 5;6:100043. doi: 10.1016/j.tcsw.2020.100043. eCollection 2020 Dec.
is an environmental, non-tuberculous mycobacterial species, capable of causing infections in humans. Biofilm formation is a key strategy used by in colonising niches in the environment and in the host. We studied a water-air interface (pellicle) biofilm of using a wide array of approaches to outline the molecular structure and composition of the biofilm. Scanning electron micrographs showed that biofilms produced an extracellular matrix. Using a combination of biochemical analysis, Raman spectroscopy, and fluorescence microscopy, we showed the matrix to consist of proteins, carbohydrates, lipids and eDNA. Glucose was the predominant sugar present in the biofilm matrix, and its relative abundance decreased in late (established) biofilms. RNA-seq analysis of the biofilms showed upregulation of genes involved in redox metabolism. Additionally, genes involved in mycolic acid, other lipid and glyoxylate metabolism were also upregulated in the early biofilms.
是一种环境性非结核分枝杆菌，能够引起人类感染。生物膜形成是该菌在环境和宿主体内定殖生态位时所采用的关键策略。我们使用多种方法研究了该菌的水-气界面（菌膜）生物膜，以勾勒生物膜的分子结构和组成。扫描电子显微镜图像显示该菌生物膜产生了细胞外基质。通过生化分析、拉曼光谱和荧光显微镜相结合的方法，我们发现该基质由蛋白质、碳水化合物、脂质和胞外DNA组成。葡萄糖是生物膜基质中存在的主要糖类，其相对丰度在后期（成熟）生物膜中降低。对生物膜的RNA测序分析表明，参与氧化还原代谢的基因上调。此外，参与分枝菌酸、其他脂质和乙醛酸代谢的基因在早期生物膜中也上调。
