Sugiyama Hironori, Osaki Toshihisa, Takeuchi Shoji, Toyota Taro
Department of Basic Science, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo 153-8902, Japan.
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8505, Japan.
ACS Omega. 2020 Jul 30;5(31):19429-19436. doi: 10.1021/acsomega.0c01371. eCollection 2020 Aug 11.
For the construction of a chemical model of contemporary living cells, the so-called water-in-oil emulsion transfer (WOET) method has drawn much attention as one of the promising preparation protocols for cell-sized liposomes encapsulating macromolecules and even micrometer-sized colloidal particles in high yields. Combining the throughput and accuracy of the observation is the key to developing a synthetic approach based on the liposomes prepared by the WOET method. Recent advances in microfluidic technology can provide a solution. By means of surface modification of a poly(dimethylsiloxane)-type microfluidic device integrating size-sorting and trapping modules, here, we enabled a simultaneous direct observation of the liposomes with a narrow size distribution, which were prepared by the WOET method. As a demonstration, we evaluated the variance of encapsulation of polystyrene colloidal particles and water permeability of the cell-sized liposomes prepared by the WOET method in the device. Since the liposomes prepared by the WOET method are useful for constructing cell models with an easy protocol, the current system will lead to a critical development of not only supramolecular chemistry and soft matter physics but also synthetic biology.
对于构建当代活细胞的化学模型而言，所谓的油包水乳液转移（WOET）方法作为一种颇具前景的制备方案，已备受关注。该方法能够高产率地制备包封大分子乃至微米级胶体粒子的细胞大小的脂质体。将观察的通量与准确性相结合，是开发基于WOET方法制备的脂质体的合成方法的关键。微流控技术的最新进展能够提供一种解决方案。在此，通过对集成了尺寸分选和捕获模块的聚二甲基硅氧烷型微流控装置进行表面改性，我们实现了对通过WOET方法制备的、具有窄尺寸分布的脂质体的同步直接观察。作为演示，我们评估了在该装置中通过WOET方法制备的细胞大小的脂质体对聚苯乙烯胶体粒子的包封差异以及水渗透性。由于通过WOET方法制备的脂质体对于以简便方案构建细胞模型很有用，当前系统不仅将推动超分子化学和软物质物理学的关键发展，还将推动合成生物学的关键发展。
