Charostad Javad, Mokhtari-Azad D V M Talat, Yavarian Jila, Ghavami Nastaran, Mahmood Seyed Khorrami Seyed, Behboudi Emad, Jalilvand Somayeh, Shatizadeh Malekshahi Somayeh, Zahra Shafiei-Jandaghi Nazanin
Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Int J Reprod Biomed. 2020 Jul 22;18(7):501-508. doi: 10.18502/ijrm.v13i7.7367. eCollection 2020 Jul.
Miscarriage is the spontaneous pregnancy loss before 24 wk of gestation. The incidence rate of miscarriage over the past few decades has shown steady or even growing trends. Viral intrauterine infections are one of the probable etiological causes of miscarriage. Previous evidence have shown that human herpes viruses (HHVs) could be considered as the potential reasons for intrauterine infections and adverse pregnancy outcomes.
This case-control study aimed to detect HHV1-5 DNAs in placental tissues and assess their association with miscarriage during the first 24 wk of pregnancy in spontaneous and therapeutic abortions.
Placental tissues from 83 women with spontaneous abortions during the first and the second trimesters of pregnancy and 81 women with therapeutic abortion during the same gestational age were collected. The DNA extraction was performed by the phenol/chloroform method. A part of the DNA polymerase gene of HHVs was amplified with multiplex nested-polymerase chain reaction. The polymerase chain reaction products were subjected to sequencing.
The results showed the presence of human cytomegalovirus genome in the placenta of both spontaneous (8.4%) and therapeutic (4.9%) abortions. No statistically significant differences were found between these two groups. The other investigated viruses were not detected here.
In conclusion, like some other studies, no correlation was detected between the HHVs placental infections and the increased risk of spontaneous abortions. In order to find the actual role of HHVs infections in miscarriage, further investigations should be performed on a larger sample size in different areas.
流产是指妊娠24周前的自然流产。在过去几十年中，流产的发生率呈稳定甚至上升趋势。病毒宫内感染是流产可能的病因之一。先前的证据表明，人类疱疹病毒（HHV）可被视为宫内感染和不良妊娠结局的潜在原因。
本病例对照研究旨在检测胎盘组织中的HHV1 - 5 DNA，并评估其与妊娠前24周自然流产和治疗性流产中流产的相关性。
收集83例妊娠前三个月和第二个月自然流产妇女以及81例相同孕周治疗性流产妇女的胎盘组织。采用酚/氯仿法进行DNA提取。用多重巢式聚合酶链反应扩增HHV的部分DNA聚合酶基因。对聚合酶链反应产物进行测序。
结果显示，自然流产（8.4%）和治疗性流产（4.9%）的胎盘组织中均存在人巨细胞病毒基因组。两组之间未发现统计学上的显著差异。此处未检测到其他所研究的病毒。
总之，与其他一些研究一样，未发现HHV胎盘感染与自然流产风险增加之间存在相关性。为了确定HHV感染在流产中的实际作用，应在不同地区对更大样本量进行进一步研究。
