Ambühl Lea M M, Leonhard Anne K, Widen Zakhary Carina, Jørgensen Annemette, Blaakaer Jan, Dybkaer Karen, Baandrup Ulrik, Uldbjerg Niels, Sørensen Suzette
Center for Clinical Research, North Denmark Regional Hospital, Hjørring, Denmark.
Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
Acta Obstet Gynecol Scand. 2017 Oct;96(10):1188-1196. doi: 10.1111/aogs.13190. Epub 2017 Aug 14.
Recently, an association between human papillomavirus infection and both spontaneous abortion and spontaneous preterm delivery was suggested. However, the reported human papillomavirus prevalence in pregnant women varies considerably and reliable conclusions are difficult. We aimed to investigate human papillomavirus infection in placental tissue of a Danish study cohort. Furthermore, we studied the cellular localization of human papillomavirus.
In this prospective case-control study, placental tissue was analyzed for human papillomavirus infection by nested PCR in the following four study groups: full-term delivery (n = 103), spontaneous preterm delivery (n = 69), elective abortion (n = 54), and spontaneous abortion (n = 44). Moreover, human papillomavirus cellular target was identified using in situ hybridization.
Human papillomavirus prevalence in placental tissue was 8.7% in full-term deliveries, 8.8% in spontaneous preterm deliveries, 10.9% in spontaneous abortions, and 20.4% in elective abortions. Twelve different human papillomavirus types were detected, and placental human papillomavirus infection was associated to a disease history of cervical cancer. Human papillomavirus DNA was identified in trophoblast cells, cells of the placental villi mesenchyme including Hofbauer cells, and in parts of the encasing endometrium.
Placental human papillomavirus infections are not likely to constitute a risk factor for spontaneous preterm labor or spontaneous abortions in the Danish population, although an effect of human papillomavirus DNA in placental cells cannot be excluded.
最近，有人提出人乳头瘤病毒感染与自然流产和自然早产之间存在关联。然而，报道的孕妇中人乳头瘤病毒感染率差异很大，难以得出可靠结论。我们旨在调查丹麦一个研究队列胎盘组织中的人乳头瘤病毒感染情况。此外，我们研究了人乳头瘤病毒的细胞定位。
在这项前瞻性病例对照研究中，通过巢式聚合酶链反应对以下四个研究组的胎盘组织进行人乳头瘤病毒感染分析：足月分娩（n = 103）、自然早产（n = 69）、人工流产（n = 54）和自然流产（n = 44）。此外，使用原位杂交鉴定人乳头瘤病毒的细胞靶点。
足月分娩胎盘组织中人乳头瘤病毒感染率为8.7%，自然早产为8.8%，自然流产为10.9%，人工流产为20.4%。检测到12种不同的人乳头瘤病毒类型，胎盘人乳头瘤病毒感染与宫颈癌病史有关。在滋养层细胞、包括霍夫鲍尔细胞在内的胎盘绒毛间充质细胞以及部分包绕的子宫内膜中鉴定出人乳头瘤病毒DNA。
在丹麦人群中，胎盘人乳头瘤病毒感染不太可能构成自然早产或自然流产的危险因素，尽管不能排除人乳头瘤病毒DNA在胎盘细胞中的作用。
