Li Yongjuan, Chen Xiaoyan, Xue Wei, Liang Junjun, Wang Liang
Department of Gastroanorectal Surgery, Anqiu People's Hospital, Weifang, 262100, China.
Department of Cardiothoracic Surgery, Anqiu People's Hospital, Weifang, 262100, China.
Neuromolecular Med. 2021 Jun;23(2):247-255. doi: 10.1007/s12017-020-08608-0. Epub 2020 Aug 14.
Previous studies showed that miR-874 expression was abnormally expressed in many tumors. However, the potential role of miR-874 in glioma remains a mystery. This study aimed to investigate its expression, clinical significance, and cellular function in glioma. A total of 105 glioma patients were enrolled in the present study. The RT-qPCR analysis was used to detect the expression of miR-874 in glioma tissues and cells. The χ test was used to analyze the association between miR-874 expression and clinical characteristics of patients. Kaplan-Meier method and multivariate Cox regression assays were used to analyze the prognostic value of miR-874 in glioma. Cell counting kit-8 and Transwell assays were used to explore the alterations in a series of cancer-related phenotypes affected by miR-874, including cell proliferation, migration, and invasion capacities. The expression of miR-874 was significantly downregulated in human glioma tissue specimens and cell lines. Furthermore, the expression of miR-874 was associated with tumor size, KPS, and WHO grade. The decreased expression of miR-874 was associated with shorter overall survival. Then functional assays indicated that upregulation of miR-874 suppressed proliferation, migration, and invasion of glioma cells in vitro. The present study indicated that miR-874 inhibited cell proliferation, migration, and invasion of glioma cells and might be a novel prognostic biomarker and potential therapeutic target for glioma.
先前的研究表明，miR-874在许多肿瘤中表达异常。然而，miR-874在胶质瘤中的潜在作用仍是个谜。本研究旨在探讨其在胶质瘤中的表达、临床意义及细胞功能。本研究共纳入105例胶质瘤患者。采用RT-qPCR分析检测miR-874在胶质瘤组织和细胞中的表达。采用χ检验分析miR-874表达与患者临床特征之间的相关性。采用Kaplan-Meier法和多因素Cox回归分析评估miR-874在胶质瘤中的预后价值。使用细胞计数试剂盒-8和Transwell实验来探究受miR-874影响的一系列癌症相关表型的变化，包括细胞增殖、迁移和侵袭能力。miR-874在人脑胶质瘤组织标本和细胞系中的表达显著下调。此外，miR-874的表达与肿瘤大小、KPS评分和世界卫生组织分级相关。miR-874表达降低与总生存期缩短有关。随后的功能实验表明，上调miR-874可抑制胶质瘤细胞在体外的增殖、迁移和侵袭。本研究表明，miR-874抑制胶质瘤细胞的增殖、迁移和侵袭，可能是一种新的预后生物标志物和胶质瘤的潜在治疗靶点。
