利用分枝杆菌静止细胞从高浓度植物甾醇中生产 22-羟基-23,24-双降胆甾-4-烯-3-酮的循环批次生物转化策略。

A recycled batch biotransformation strategy for 22-hydroxy-23,24-bisnorchol-4-ene-3-one production from high concentration of phytosterols by mycobacterial resting cells.

机构信息

State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

出版信息

Biotechnol Lett. 2020 Dec;42(12):2589-2594. doi: 10.1007/s10529-020-02991-1. Epub 2020 Aug 17.

DOI:10.1007/s10529-020-02991-1

PMID:32804273

Abstract

OBJECTIVES

To realize a practical technology for recycling both cyclodextrin and resting-cells at the same time in phytosterol biotransformation using mycobacterial resting cells.

RESULTS

In order to produce 22-hydroxy-23,24-bisnorchol-4-ene-3-one (HBC) efficiently and low-costly, a recycled phytosterols (PS) biotransformation process using mycobacterial resting cells was developed. By optimizing the ratio of hydroxypropyl-β-cyclodextrin (HP-β-CD) and PS to 1:1 (w/w), most products crystallized during the biotransformation process. So, the HBC was easily separated by low-speed (900×g) centrifugation with yield of 92%. The resting cells, HP-β-CD and the residual products and substrates left in the reaction system were reused for another bioconversion cycle after PS supplement. Three continuous cycles were achieved without the supplement of cells and HP-β-CD. In each batch, 80 g L of PS was transformed to HBC with the space-time yield of HBC of 8.9-12.8 g L day.

CONCLUSIONS

This strategy reduced the cost of HBC production and simplified the purification process.

摘要

目的

利用分枝杆菌休眠细胞，实现同时回收环糊精和休眠细胞的实用技术，用于植物甾醇生物转化。

结果

为了高效、低成本地生产 22-羟基-23,24-双降胆甾-4-烯-3-酮（HBC），开发了一种利用分枝杆菌休眠细胞的可再生植物甾醇（PS）生物转化工艺。通过优化羟丙基-β-环糊精（HP-β-CD）和 PS 的比例为 1:1（w/w），大多数产物在生物转化过程中结晶。因此，HBC 可以通过低速（900×g）离心轻松分离，收率为 92%。休眠细胞、HP-β-CD 和反应系统中剩余的产物和底物在 PS 补充后可重复用于另一个生物转化循环。在没有补充细胞和 HP-β-CD 的情况下，进行了三个连续循环。在每个批次中，80 g L 的 PS 转化为 HBC，HBC 的时空产率为 8.9-12.8 g L day。