Chen Lu, Lian Hui-Ting, Sun Xiang-Ying, Liu Bin
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, People's Republic of China.
College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, People's Republic of China.
Anal Biochem. 2017 Jun 1;526:58-65. doi: 10.1016/j.ab.2017.03.017. Epub 2017 Mar 19.
A novel electrochemical sensor was presented for the determination of L-5-hydroxytryptophan (L-5-HTP) based on a graphene-chitosan molecularly imprinted film modified on the surface of glassy carbon electrode (GR-MIP/GCE). The morphology and composition of the imprinted film were observed in field emission scanning electron microscopy (FESEM), raman spectroscopy and fourier transform infrared (FTIR). The properties of the sensor were evaluated by electrochemical techniques. Under the optimal conditions, the peak currents of L-5-HIP were found to be linear in the concentration range of 0.05-7.0 μM, while the sensor also exhibited great features such as low detection limit of 6.0 nM (S/N = 3), superb selectivity against the structural analogues, good antidisturbance ability among coexisting components, excellent repeatability and stability. Moreover, the proposed method had been applied to the detection of L-5-HTP in human blood serum with a satisfactory recoveries ranging from 90.6% to 105.6%.
提出了一种基于修饰在玻碳电极（GR-MIP/GCE）表面的石墨烯-壳聚糖分子印迹膜测定L-5-羟色氨酸（L-5-HTP）的新型电化学传感器。用场发射扫描电子显微镜（FESEM）、拉曼光谱和傅里叶变换红外光谱（FTIR）观察了印迹膜的形态和组成。通过电化学技术评估了传感器的性能。在最佳条件下，发现L-5-HIP的峰电流在0.05-7.0 μM浓度范围内呈线性，同时该传感器还具有低检测限6.0 nM（S/N = 3）、对结构类似物具有极佳的选择性、在共存组分中具有良好的抗干扰能力、出色的重复性和稳定性等优异特性。此外，所提出的方法已应用于人血清中L-5-HTP的检测，回收率令人满意，在90.6%至105.6%之间。
