5'-三磷酸-1-β-D-阿拉伯呋喃糖基胞嘧啶对小牛胸腺DNA聚合酶α和β的抑制作用。
Inhibition of DNA polymerase-alpha and -beta of calf thymus by 1-beta-D-arabinofuranosylcytosine-5'-triphosphate.
作者信息
Yoshida S, Yamada M, Masaki S
出版信息
Biochim Biophys Acta. 1977 Jul 15;477(2):144-50. doi: 10.1016/0005-2787(77)90230-1.
1-beta-D-Arabinofuranosylcytosine 5'-triphosphate (araCTP), an active form of a inhibitor of DNA replication, 1-beta-D-arabinofuranosylcytosine (araC) was tested for its inhibitory action on the DNA polymerase-alpha and -beta (EC 2.7.7.7) purified from calf thymus. The reaction of DNA polymerase-alpha was shown to be more sensitive to the inhibition by araCTP than that of DNA polymerase-beta. The mode of the inhibition by araCTP was competitive to dCTP in the reaction catalysed by either DNA polymerase-alpha or -beta. The Ki value of DNA polymerase-beta for araCTP was 32 micron; eight times higher than that of DNA polymerase-alpha (4 micron) for this inhibition.
1-β-D-阿拉伯呋喃糖基胞嘧啶5'-三磷酸（araCTP）是一种DNA复制抑制剂的活性形式，对1-β-D-阿拉伯呋喃糖基胞嘧啶（araC）针对从 calf胸腺中纯化的DNA聚合酶α和β（EC 2.7.7.7）的抑制作用进行了测试。结果显示，DNA聚合酶α的反应比DNA聚合酶β的反应对araCTP的抑制更敏感。在由DNA聚合酶α或β催化的反应中，araCTP的抑制模式与dCTP竞争。DNA聚合酶β对araCTP的Ki值为32微摩尔；比DNA聚合酶α（4微摩尔）对此抑制作用的Ki值高八倍。
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