2',3'-二脱氧-3'-氨基核苷5'-三磷酸是DNA聚合酶催化的DNA合成的终止剂。

Chidgeavadze Z G, Beabealashvilli R S, Atrazhev A M, Kukhanova M K, Azhayev A V, Krayevsky A A

Nucleic Acids Res. 1984 Feb 10;12(3):1671-86. doi: 10.1093/nar/12.3.1671.

It is shown that 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates with adenine, guanine, cytosine and thymine bases are effective inhibitors of DNA polymerase I, calf thymus DNA polymerase alpha and rat liver DNA polymerase beta. The effect of the above-mentioned compounds is markedly higher than corresponding action of the well-known DNA synthesis inhibitors arabinonucleoside 5'-triphosphates and 2',3'-dideoxynucleoside 5'-triphosphates. 2',3'-dideoxy-3'-aminonucleoside 5'-monophosphate residues incorporate into the 3'-terminus of the primer and terminate the DNA chain elongation. The possibility of using 2',3'-dideoxy-3'-aminonucleoside 5'-triphosphates as terminators for DNA sequencing by the polymerization method is demonstrated.

结果表明，带有腺嘌呤、鸟嘌呤、胞嘧啶和胸腺嘧啶碱基的2'，3'-二脱氧-3'-氨基核苷5'-三磷酸是DNA聚合酶I、小牛胸腺DNA聚合酶α和大鼠肝脏DNA聚合酶β的有效抑制剂。上述化合物的作用明显高于著名的DNA合成抑制剂阿拉伯核苷5'-三磷酸和2'，3'-二脱氧核苷5'-三磷酸的相应作用。2'，3'-二脱氧-3'-氨基核苷5'-单磷酸残基掺入引物的3'-末端并终止DNA链的延伸。证明了使用2'，3'-二脱氧-3'-氨基核苷5'-三磷酸作为聚合方法进行DNA测序的终止剂的可能性。