Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, IPN, Ciudad de México, Mexico; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, Mexico.
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, IPN, Ciudad de México, Mexico.
Dev Comp Immunol. 2021 Jan;114:103830. doi: 10.1016/j.dci.2020.103830. Epub 2020 Aug 15.
Different evidences suggest that pericardial cells play an important role during the immune response against pathogens that invade the mosquito hemocoel. Previously, we identified two lysozyme genes in Anopheles albimanus heart transcriptome. The present study showed that one of these genes (ID: AALB004517) has high percentage of identity to mosquito lysozyme genes related to immunity, suggesting its possible participation during the mosquito immune response. This An. albimanus gen, constitutively expressed lysozyme c-1 mRNA (albLys c-1) in mosquito heart; however, it was overexpressed in bacteria-injected mosquitoes. In heart extract samples, we identified a protein of approximately 14 kDa (likely lysozyme c-1), which lysed M. luteus. In addition, mRNA-FISH assay in heart samples, showed specific fluorescent hybridization signal in pericardial cells from M. luteus-injected mosquitos. We conclude that for the first time an inducible immune factor (lysozyme c-1) is identified in Anopheles albimanus mosquito pericardial cells, which could be a key component in the response against pathogens that interact with the mosquito heart.
不同的证据表明，在心包细胞在抵抗入侵蚊虫血腔的病原体的免疫反应中起着重要作用。先前，我们在按蚊心脏转录组中鉴定了两个溶菌酶基因。本研究表明，其中一个基因（ID：AALB004517）与与免疫相关的蚊子溶菌酶基因具有很高的同源性，表明其可能参与蚊子的免疫反应。该基因在蚊子心脏中组成性表达溶菌酶 c-1 mRNA（albLys c-1）；然而，在注射细菌的蚊子中其表达量上调。在心组织提取样本中，我们鉴定到一种约 14 kDa 的蛋白（可能是溶菌酶 c-1），它可以溶解 M. luteus。此外，在心组织样本中的 mRNA-FISH 检测显示，在注射 M. luteus 的蚊子的心包细胞中存在特异性荧光杂交信号。我们的结论是，首次在按蚊心包细胞中鉴定到一种诱导型免疫因子（溶菌酶 c-1），它可能是与蚊子心脏相互作用的病原体的反应中的关键组成部分。
