Centro de Investigaciones Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Col. Sta. María Ahuacatitlan, Cuernavaca, Morelos, México.
Cell Tissue Res. 2013 Jan;351(1):127-37. doi: 10.1007/s00441-012-1505-6. Epub 2012 Nov 16.
Insect pericardial cells (PCs) are strategically located along the dorsal vessel where they encounter a high hemolymph flow enabling them to undertake their osmoregulatory, detoxifying, and scavenging functions. In this location, PCs also encounter foreign molecules and microorganisms. The response of PCs of the mosquito Anopheles albimanus, one of the most important Plasmodium vivax vectors in Mexico and Latin America, to Saccharomyces cerevisiae was analyzed by using biochemical, cellular, ultrastructural, and bioinformatics approaches. Immune gene transcripts were identified in the PC transcriptome of A. albimanus. PCs responded to the presence of yeast and zymosan with increased lysosomal and phosphatase activities and produced lytic activity against bacteria. Our results indicate that mosquito PCs play a key role in the neutralization and elimination of pathogens.
昆虫心肌细胞（PCs）位于背血管的战略位置，在那里它们遇到高血液流动，使它们能够进行渗透调节、解毒和清除功能。在这个位置，PCs 还会遇到外来分子和微生物。本研究采用生物化学、细胞、超微结构和生物信息学方法，分析了蚊子 Anopheles albimanus 心肌细胞对酿酒酵母的反应，Anopheles albimanus 是墨西哥和拉丁美洲最重要的间日疟原虫传播媒介之一。在 A. albimanus 的 PC 转录组中鉴定出了免疫基因转录本。PCs 在酵母和酵母聚糖存在的情况下，溶酶体和磷酸酶的活性增加，并对细菌产生裂解活性。我们的结果表明，蚊子的 PCs 在中和和消除病原体方面起着关键作用。
