Liu H M, Schmid K
Department of Pathology (Neuropathology), Miriam Hospital, Providence, Rhode Island 02906.
In Vitro Cell Dev Biol. 1988 Mar;24(3):205-10. doi: 10.1007/BF02623548.
A method is described for the quantitative analysis of the nerve-growth-promoting activity of biological molecules in tissue culture. The criteria used for the evaluation of this activity is based on the neurite length as well as the total number of neurites produced by the explant of whole dorsal root ganglia from 12-d-old chick embryos. A nerve growth index (NGI) is given to each ganglion during each of a 5-d culture period. The NGI is defined as the product of average neurite length in millimeters and the total number of neurites. We report that with increasing concentrations of fetal bovine serum, there was a proportional increase in NGI due to increased neurite density while the neurite length was not greatly affected. The NGI of several proteins with known nerve growth promoting activity, namely nerve growth factor, insulin, transferrin, and fibronectin were investigated for their activity and compared with that of fetal bovine serum.
本文描述了一种用于定量分析组织培养中生物分子促神经生长活性的方法。评估该活性所使用的标准基于神经突长度以及来自12日龄鸡胚的整个背根神经节外植体产生的神经突总数。在为期5天的培养期内，每天为每个神经节给出一个神经生长指数（NGI）。NGI定义为以毫米为单位的平均神经突长度与神经突总数的乘积。我们报告，随着胎牛血清浓度的增加，由于神经突密度增加，NGI成比例增加，而神经突长度未受到很大影响。研究了几种具有已知促神经生长活性的蛋白质，即神经生长因子、胰岛素、转铁蛋白和纤连蛋白的NGI，并将其活性与胎牛血清的活性进行了比较。
