School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110006, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110006, China; Liaoning Provincial Key Laboratory of TCM Resources Conservation and Development, Shenyang 110006, China.
Bioresour Technol. 2020 Dec;317:123945. doi: 10.1016/j.biortech.2020.123945. Epub 2020 Jul 31.
The purpose of this study was to determine the production of sugars from ginseng residues treated with laccase. Laccase was used to degrade lignin from ginseng residues in order to increase the yield of sugars. Reaction conditions, including solid loading, pH, enzyme concentration, incubation temperature, and incubation time, were investigated and optimized. The results showed that the optimum conditions were 20% of solid loading (w/v), pH 7, 300 IU/ml, temperature of 40 °C and incubation time of 6 h. The minimum residual lignin obtained was 59.89%. The results also showed that 56.58% sugars including 12.04% water soluble polysaccharides (WSP), 16.24% water insoluble polysaccharides (WIP) and 5.08% reducing sugar were afforded from delignify substance. Chemical characters of these sugars were analyzed. Pretreat of laccase delignification for sugars production is expected to be applied to other herbal residues.
本研究旨在确定用漆酶处理人参残渣生产糖的情况。漆酶用于降解人参残渣中的木质素，以提高糖的产量。考察并优化了反应条件，包括固载量、pH 值、酶浓度、孵育温度和孵育时间。结果表明，最佳条件为固载量（w/v）20%、pH 值 7、300IU/ml、温度 40°C 和孵育时间 6 h。获得的最小残余木质素为 59.89%。结果还表明，从脱木质素物质中得到了 56.58%的糖，包括 12.04%水溶性多糖（WSP）、16.24%水不溶性多糖（WIP）和 5.08%还原糖。分析了这些糖的化学性质。预计漆酶预处理脱木质素法将应用于其他草药残渣的糖生产。
