Advanced Technology Development Centre, Indian Institute of Technology, Kharagpur, 721302, India.
Department of Civil Engineering, Indian Institute of Technology, Kharagpur, 721302, India.
J Environ Manage. 2018 Jul 1;217:700-709. doi: 10.1016/j.jenvman.2018.04.008. Epub 2018 Apr 24.
Bioethanol production from lignocellulosic biomass is a promising approach towards finding an alternative for transportation fuels that is driven by the prerequisite to lessen our dependency on fossil fuels, increase energy security and mitigate greenhouse gas emission. Recalcitrance of lignocellulosic biomass is a major hindrance in bioethanol production. Hence, an efficient pretreatment method is necessary for degradation of lignin and providing accessibility of holocellulose for hydrolysis. In an attempt to overcome this bottleneck, laccase mediated delignification of sugarcane tops was studied using central composite design (CCD) based on response surface methodology (RSM). The effect of different process parameters such as temperature, pH, solid loading, enzyme titre and incubation time were evaluated. It was observed that under optimum conditions of pH 7, solid loading of 21% (w/v), enzyme titre of 430.3 IU/mL, temperature of 40 °C and incubation of 6 h, maximum delignification of 79.1% was achieved. Compositional analysis, energy density measurement and water retention capacity of the biomass was also conducted along with GC-MS analysis for identification of low molecular compounds formed during delignification. Structural characterization of the biomass before and after pretreatment process were analysed by Scanning Electron Microscopy (SEM), Fourier-Transform Infra-Red Spectroscopy (FTIR) and X-Ray Diffraction Spectroscopy (XRD) that further substantiated the delignification of sugarcane tops.
从木质纤维素生物质生产生物乙醇是寻找替代运输燃料的一种有前途的方法，这是因为我们需要减少对化石燃料的依赖、提高能源安全性和减少温室气体排放。木质纤维素生物质的顽固性是生物乙醇生产的主要障碍。因此，需要一种有效的预处理方法来降解木质素，并为全纤维素的水解提供可及性。为了克服这一瓶颈，使用基于响应面法（RSM）的中心组合设计（CCD）研究了漆酶介导的甘蔗梢脱木质素。评估了不同工艺参数（如温度、pH 值、固载量、酶活和孵育时间）的影响。结果表明，在 pH 值为 7、固载量为 21%（w/v）、酶活为 430.3 IU/mL、温度为 40°C 和孵育 6 h 的最佳条件下，脱木质素率达到了 79.1%。还对生物质进行了成分分析、能量密度测量和保水能力测试，并进行了 GC-MS 分析以鉴定脱木质素过程中形成的低分子化合物。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线衍射光谱（XRD）对预处理前后生物质的结构特征进行了分析，进一步证实了甘蔗梢的脱木质素。
