Chinese Academy of Sciences, Chongqing Institute of Green and Intelligent Technology, Chongqing 400714, PR China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
Bioresour Technol. 2020 Dec;317:123976. doi: 10.1016/j.biortech.2020.123976. Epub 2020 Aug 8.
The aim of this work was to study the bioaugmentation of P. stutzeri strain XL-2 in activated sludge to improve nitrogn removal from wastewater with the guide of growth kinetics. When 4250 mg/L COD and 80 mg/L NH-N were applied, the TN removal efficiency in a bioaugmented sequencing batch reactor (SBR) achieved 95%, while that in the control reactor (SBR) without strain XL-2 was only 84% (P < 0.05). The microbial community analysis demonstrated that strain XL-2 was successfully bioaugmented in SBR, and increasing influent COD concentration promoted its abundance. Influent COD concentration played a dominant role in affecting community structure, while the bioaugmentation of strain XL-2 had much less impact on the community structure. Combined with principal coordinates analysis, redundancy analysis and FAPROTAX, the improvement of TN removal was mainly achieved by the bioaugmentation of strain XL-2, which played a major role in promoting aerobic denitrification.
本工作旨在通过生长动力学指导，研究在活性污泥中对 P. stutzeri 菌株 XL-2 进行生物强化，以提高废水中的氮去除率。当应用 4250mg/L COD 和 80mg/L NH-N 时，生物强化序批式反应器（SBR）中的 TN 去除效率达到 95%，而没有 XL-2 菌株的对照反应器（SBR）仅为 84%（P<0.05）。微生物群落分析表明，XL-2 菌株在 SBR 中成功得到生物强化，并且增加进水 COD 浓度促进了其丰度的增加。进水 COD 浓度对群落结构的影响起主导作用，而 XL-2 菌株的生物强化对群落结构的影响较小。结合主坐标分析、冗余分析和 FAPROTAX，TN 去除的改善主要是通过 XL-2 菌株的生物强化实现的，该菌株在促进好氧反硝化方面发挥了主要作用。
