School of Materials and Chemical Engineering, Zhongyuan University of Technology, Zhengzhou 450007, P. R. China.
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, P. R. China.
ACS Appl Mater Interfaces. 2020 Sep 9;12(36):40153-40162. doi: 10.1021/acsami.0c13237. Epub 2020 Aug 25.
This paper proposes a highly efficient antibacterial system based on a synergistic combination of photodynamic therapy, photothermal therapy, and chemotherapy. Chitosan oligosaccharide functionalized graphene quantum dots (GQDs-COS) with short-term exposure to 450 nm visible light are used to promote rapid healing in bacteria-infected wounds. The GQDs undergo strong photochemical transformation to rapidly produce radical oxygen species and heat under light illumination, while the COS has an innate antimicrobial ability. Moreover, the positively charged GQDs-COS can easily capture bacteria via electrostatic interactions and kill Gram-positive and Gram-negative bacteria by multivalent interactions and synergistic effects. The antibacterial action of this nanocomposite causes irreversible damage to outer and inner bacterial membranes, resulting in cytoplasm leakage and death. The system has good hemocompatibility and low cytotoxicity and can improve the healing of infected wounds, as demonstrated by the examination of pathological tissue sections and inflammatory markers. These results suggest that GQDs anchored with bioactive molecules are a potential photo-activated antimicrobial strategy for anti-infective therapy.
本文提出了一种基于光动力疗法、光热疗法和化学疗法协同组合的高效抗菌系统。壳聚糖寡糖功能化石墨烯量子点（GQDs-COS）在 450nm 可见光下短时间暴露，可促进细菌感染伤口的快速愈合。GQDs 在光照射下经历强烈的光化学反应，迅速产生自由基氧和热，而 COS 具有先天的抗菌能力。此外，带正电荷的 GQDs-COS 可以通过静电相互作用轻松捕获细菌，并通过多价相互作用和协同效应杀死革兰氏阳性菌和革兰氏阴性菌。该纳米复合材料的抗菌作用会对细菌的内外膜造成不可逆转的损伤，导致细胞质泄漏和死亡。该系统具有良好的血液相容性和低细胞毒性，可以改善感染伤口的愈合，这可以通过检查病理组织切片和炎症标志物来证明。这些结果表明，锚定生物活性分子的 GQDs 是一种用于抗感染治疗的潜在光激活抗菌策略。
