Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Department of Ceramic Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi 221005, India.
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111138. doi: 10.1016/j.msec.2020.111138. Epub 2020 Jun 1.
In the present study, the combined effect of addition of varying concentrations (10-30 vol%) of biocompatible piezoelectric NaKNbO (NKN) as well as electrostatic and dynamic pulsed electrical treatment on antibacterial and cellular response of 1393 bioactive glass (1393 BG) has been examined. The phase analyses of the sintered (at 800 °C for 30 min) samples revealed the formation of 1393 BG - NKN composites without any appearance of secondary phases. The addition of 10-30 vol% NKN significantly improved the mechanical behaviour of 1393 BG like, hardness (1.7 to 2 times), fracture toughness (1.3 to 2.6 times), compressive (2.3 to 8 times) and flexural strengths (2 to 3.5 times) than monolithic 1393 BG. The piezoelectric NKN is observed to induce the antibacterial activity in 1393 BG - (10- 30 vol%) NKN composites, while Staphylococcus aureus (S. aureus, gram positive) and Escherichia coli (E. coli, gram negative) bacterial cells were exposed to unpolarized and polarized (20 kV, 500°C for 30 min) sample surfaces. The antibacterial response was examined using disc diffusion, nitro blue tetrazolium (NBT) and MTT assays. The statistical analyses revealed the significant reduction in the viability of bacterial cells on polarized 1393 BG - (10- 30 vol%) NKN composite samples. In addition, the combined effect of electrostatic and dynamic pulsed electrical stimulation (1 V/cm, 500 μs pulses) on the cellular response of 1393 BG and 1393 BG - 30 vol% NKN composites has been analysed with MG-63 osteoblast-like cells. The cell proliferation was observed to increase significantly for the dynamic pulsed electric field treated negatively charged surfaces.
在本研究中，考察了添加不同浓度（10-30vol%）生物相容性压电 NaKNbO（NKN）以及静电和动态脉冲电处理对 1393 生物活性玻璃（1393BG）的抗菌和细胞反应的综合影响。对烧结（800°C 30min）样品的相分析表明，形成了 1393BG-NKN 复合材料，没有出现任何第二相。添加 10-30vol%NKN 显著提高了 1393BG 的力学性能，如硬度（提高 1.7 至 2 倍）、断裂韧性（提高 1.3 至 2.6 倍）、抗压强度（提高 2 至 8 倍）和抗弯强度（提高 2 至 3.5 倍），优于整体 1393BG。观察到压电 NKN 可在 1393BG-（10-30vol%）NKN 复合材料中诱导抗菌活性，而金黄色葡萄球菌（金黄色葡萄球菌，革兰氏阳性）和大肠杆菌（大肠杆菌，革兰氏阴性）细菌细胞暴露于非极化和极化（20kV，500°C 30min）样品表面。使用圆盘扩散、硝基蓝四唑（NBT）和 MTT 测定法检查抗菌反应。统计分析表明，极化 1393BG-（10-30vol%）NKN 复合材料样品上细菌细胞的活力显著降低。此外，还分析了静电和动态脉冲电刺激（1V/cm，500μs 脉冲）对 1393BG 和 1393BG-30vol%NKN 复合材料细胞反应的综合影响。观察到在带负电荷的表面上用动态脉冲电场处理后细胞增殖显著增加。
