Centre for Environmental and Marine Studies (CESAM), Campus de Santiago, Universidade de Aveiro, 3810-193, Aveiro, Portugal.
Centre for Environmental and Marine Studies (CESAM), Campus de Santiago, Universidade de Aveiro, 3810-193, Aveiro, Portugal.
Environ Pollut. 2020 Oct;265(Pt B):114993. doi: 10.1016/j.envpol.2020.114993. Epub 2020 Jun 11.
Among the most used non-steroidal anti-inflammatory drugs (NSAIDs), ketoprofen (KTF) assumes an important position. Nevertheless, its ecotoxicological effects in non-target organisms are poorly characterized, despite its use and frequency of occurrence in aquatic matrices. Thus, the aim of this study was to evaluate the possible toxicological effects of KTF contamination, in two freshwater species, Lemna minor and Daphnia magna, by measuring biochemical, physiological and population parameters. To attain this objective, both species were exposed to KTF at the same concentrations (0, 0.24, 1.2, 6 and 30 μg/L). L. minor plants were exposed during 4 d to these levels of KTF, and the enzymatic activity (catalase (CAT), glutathione S-transferases (GSTs) and carbonic anhydrase (CA)), and pigments content (chlorophylls a, b and total and carotenoids) were analyzed to evaluate the toxicity of this drug. D. magna was acutely and chronically exposed to KTF, and enzymatic activities (CAT, GSTs and cyclooxygenase (COX)), the feeding rates, and reproduction traits were assessed. In L.minor, KTF provoked alterations in all enzyme activities, however, it was not capable of causing any alteration in any pigment levels. On the other hand, KTF also provoked alterations in all enzymatic activities in D. magna, but did not affect feeding rates and life-history parameters. In conclusion, exposure to KTF, provoked biochemical alterations in both species. However, these alterations were not reflected into deleterious effects on physiological and populational traits of L. minor and D. magna.
在最常用的非甾体抗炎药（NSAIDs）中，酮洛芬（KTF）占有重要地位。然而，尽管它在水生基质中的使用频率和出现频率都很高，但它对非靶标生物的生态毒理学效应仍知之甚少。因此，本研究旨在通过测量生化、生理和种群参数，评估 KTF 污染对两种淡水物种——浮萍（Lemna minor）和大型溞（Daphnia magna）可能产生的毒理学影响。为了实现这一目标，将这两种物种暴露于相同浓度（0、0.24、1.2、6 和 30μg/L）的 KTF 中。浮萍植物在这些 KTF 浓度下暴露了 4 天，然后分析其酶活性（过氧化氢酶（CAT）、谷胱甘肽 S-转移酶（GSTs）和碳酸酐酶（CA））和色素含量（叶绿素 a、b 和总类胡萝卜素），以评估该药物的毒性。大型溞急性和慢性暴露于 KTF 后，评估其酶活性（CAT、GSTs 和环氧化酶（COX））、摄食率和繁殖特征。在浮萍中，KTF 引起了所有酶活性的改变，但未能引起任何色素水平的改变。另一方面，KTF 也引起了大型溞所有酶活性的改变，但未影响摄食率和生活史参数。总之，暴露于 KTF 会引起两种物种的生化变化。然而，这些变化并未反映在浮萍和大型溞的生理和种群特征上产生有害影响。
