State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
Genes (Basel). 2020 Aug 12;11(8):927. doi: 10.3390/genes11080927.
Plant-specific NAC (, , ) transcription factor (TF) family plays important roles in biological processes such as plant growth and response to stress. Nevertheless, no information is known about NAC TFs in , a prominent xerophyte desert grass in northwestern China. In this study, 162 genes were found from the genome, among which 156 () genes (96.3%) were mapped onto 20 chromosomes. The phylogenetic tree constructed by CsNAC and rice NAC TFs can be separated into 14 subfamilies. Syntenic and Ka/Ks analyses showed that were primarily expanded by genomewide replication events, and purifying selection was the primary force driving the evolution of family genes. The gene expression profiles showed that 36 genes showed differential expression between cleistogamous (CL) and chasmogamous (CH) flowers. One hundred and two genes showed differential expression under heat, cold, drought, salt and ABA treatment. Twenty-three genes were commonly differentially expressed both under stress responses and during dimorphic floret development. Gene Ontology (GO) annotation, coexpression network and qRT-PCR tests revealed that these genes may simultaneously regulate dimorphic floret development and the response to stress. Our results may help to characterize the NAC transcription factors in and provide new insights into the functional research and application of the NAC family in crop improvement, especially in dimorphic floret plants.
植物特有的 NAC（NAM、ATAF1/2、CUC2）转录因子（TF）家族在植物生长和应对胁迫等生物学过程中发挥着重要作用。然而，在中国西北地区一种重要的旱生荒漠草本植物 中，NAC TF 的相关信息尚未可知。在本研究中，从 基因组中发现了 162 个基因，其中 156 个（96.3%）被映射到 20 条染色体上。由 CsNAC 和水稻 NAC TFs 构建的系统发育树可以分为 14 个亚家族。共线性和 Ka/Ks 分析表明， 主要通过全基因组复制事件扩张，而纯化选择是驱动 家族基因进化的主要力量。 基因表达谱显示，36 个基因在闭锁花（CL）和开花花（CH）之间表现出差异表达。102 个基因在热、冷、干旱、盐和 ABA 处理下表现出差异表达。23 个基因在胁迫响应和二型小花发育过程中均表现出共同的差异表达。基因本体（GO）注释、共表达网络和 qRT-PCR 测试表明，这些 基因可能同时调节二型小花的发育和对胁迫的反应。我们的研究结果可能有助于鉴定 中的 NAC 转录因子，并为 NAC 家族在作物改良中的功能研究和应用提供新的见解，特别是在二型小花植物中。
