Martin-Rivilla Helena, Gutierrez-Mañero F Javier, Gradillas Ana, P Navarro Miguel O, Andrade Galdino, Lucas José A
Plant Physiology Pharmaceutical and Health Sciences Department, Faculty of Pharmacy, Universidad San Pablo-CEU Universities, 28668 Madrid, Spain.
Centre for Metabolomics and Bioanalyses, Faculty of Pharmacy, Universidad San Pablo-CEU Universities, 28668 Madrid, Spain.
Plants (Basel). 2020 Aug 12;9(8):1020. doi: 10.3390/plants9081020.
In this work, the metabolic elicitors extracted from the beneficial rhizobacterium N 21.4 were sequentially fragmented by vacuum liquid chromatography to isolate, purify and identify the compounds responsible for the extraordinary capacities of this strain to induce systemic resistance and to elicit secondary defensive metabolism in diverse plant species. To check if the fractions sequentially obtained were able to increase the synthesis of isoflavones and if, therefore, they still maintained the eliciting capacity of the live strain, rapid and controlled experiments were done with soybean seeds. The optimal action concentration of the fractions was established and all of them elicited isoflavone secondary metabolism-the fractions that had been extracted with n-hexane being more effective. The purest fraction was the one with the highest eliciting capacity and was also tested in seedlings to induce systemic resistance against the pathogen pv. tomato DC 3000. This fraction was then analyzed by UHPLC/ESI-QTOF-MS, and an alkaloid, two amino lipids, three arylalkylamines and a terpenoid were tentatively identified. These identified compounds could be part of commercial plant inoculants of biological and sustainable origin to be applied in crops, due to their potential to enhance the plant immune response and since many of them have putative antibiotic and/or antifungal potential.
在这项工作中，从有益根际细菌N 21.4中提取的代谢激发子通过真空液相色谱法进行顺序分离，以分离、纯化和鉴定负责该菌株在多种植物物种中诱导系统抗性和引发次生防御代谢非凡能力的化合物。为了检查顺序获得的馏分是否能够增加异黄酮的合成，以及它们是否仍保持活菌株的激发能力，对大豆种子进行了快速且可控的实验。确定了馏分的最佳作用浓度，所有馏分均引发了异黄酮次生代谢——用正己烷提取的馏分更有效。纯度最高的馏分具有最高的激发能力，也在幼苗中进行了测试，以诱导对病原菌番茄致病变种DC 3000的系统抗性。然后通过超高效液相色谱/电喷雾电离-四极杆飞行时间质谱对该馏分进行分析，初步鉴定出一种生物碱、两种氨基脂质、三种芳基烷基胺和一种萜类化合物。这些已鉴定的化合物可能成为用于作物的具有生物和可持续来源的商业植物接种剂的一部分，因为它们具有增强植物免疫反应的潜力，而且其中许多具有推定的抗生素和/或抗真菌潜力。
