Facultad de Farmacia, Universidad San Pablo CEU, Ctra. Boadilla del Monte km 5.3, 28668 Madrid, Spain.
J Agric Food Chem. 2012 Nov 7;60(44):11080-7. doi: 10.1021/jf303334q. Epub 2012 Oct 24.
Phytopharmaceuticals are plant secondary metabolites that are strongly inducible and especially sensitive to biotic changes. Plant cell cultures are a good alternative to obtain secondary metabolites, in case effective stimulation can be achieved. In this study, metabolic elicitors from two rhizobacteria able to enhance isoflavone content in soybean seedlings were tested on three different soybean calli cell lines. Results show that metabolic elicitors from Chryseobacterium balustinum Aur9 were not effective. However, there are at least two different metabolic elicitors from Pseudomonas fluorescens N21.4, one under 10 kDa and another over 10 kDa, that trigger isoflavone metabolism in the three cell lines with different isoflavone content. Elicitors from N21.4 achieved total isoflavone increases up to 29.7% (0.205 mg/g), 64.5% (0.487 mg/g), and 23.4% (0.726 mg/g) in the low-, intermediate-, and high-yield lines, respectively. Therefore, these elicitors have a great potential to enhance isoflavone production in cell cultures for development of functional ingredients.
植物药是植物次生代谢产物，对生物变化具有很强的诱导性和特别的敏感性。植物细胞培养是获得次生代谢产物的一种很好的替代方法，因为可以实现有效的刺激。在这项研究中，测试了两种能够提高大豆幼苗异黄酮含量的根际细菌的代谢诱导剂，它们分别作用于三种不同的大豆愈伤组织细胞系。结果表明，蜡状芽孢杆菌 Aur9 的代谢诱导剂没有效果。然而，荧光假单胞菌 N21.4 至少有两种不同的代谢诱导剂，一种在 10 kDa 以下，另一种在 10 kDa 以上，它们能够在三种异黄酮含量不同的细胞系中触发异黄酮代谢。N21.4 的诱导剂使低、中、高产系的总异黄酮含量分别增加了 29.7%（0.205mg/g）、64.5%（0.487mg/g）和 23.4%（0.726mg/g）。因此，这些诱导剂在细胞培养中具有很大的潜力，可以提高异黄酮的产量，用于功能性成分的开发。
