The Effect of Temperature on the Hypersensitive Response (HR) in the Pathosystem.

Temperature is considered one of the crucial environmental elements in plant pathological interactions, and previous studies have indicated that there is a relationship between temperature change and host-pathogen interactions. The objective of this research is to investigate the link between temperature and the incompatible interactions of the host and pathogen. In this study, two isolates (HCRT75 8-1 and HCRT77 7-2) and two genotypes (Surpass400 and 01-23-2-1) were selected. The selected genotypes displayed intermediate and resistant phenotypes. The inoculated seedlings were tested under three temperature conditions: 16 °C/10 °C, 22 °C/16 °C and 28 °C/22 °C (day/night: 16 h/8 h). Lesion measurements demonstrated that the necrotic lesions from the 28 °C/22 °C treatment were enlarged compared with the other two temperature treatments (i.e., 16 °C/10 °C and 22 °C/16 °C). The results of expression analysis indicated that the three temperature treatments displayed distinct differences in two marker genes ( and ) for plant defense and one temperature-sensitive gene (). Additionally, seven dpi at 22 °C/16 °C appeared to be the optimal pre-condition for the induction of and . These findings suggest that responds to temperature changes when infected with .