Ziaei Amiri Fereshteh, Pashandi Zaiddodine, Lotfibakhshaiesh Nasrin, Mirzaei-Parsa Mohamad Javad, Ghanbari Hossein, Faridi-Majidi Reza
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Tissue Engineering and Applied Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Int J Artif Organs. 2021 Mar;44(3):199-207. doi: 10.1177/0391398820947737. Epub 2020 Aug 17.
Since collagen is naturally a main extracellular matrix protein, it has been applied widely in skin's tissue engineering scaffolds to mimics the characteristics of extracellular matrix for proper transplantation of living cells. However, there are challenges that come with application of this natural polymer such as high solubility in aqueous environments which requires further consideration such as chemically cross-linking in order to stabilization. But these treatments also affect its functionality and finally cellular behaviors on scaffold. In this research we evaluated the suitability of collagen nanofibers versus collagen nanoparticles for cell adhesion and viability on glutaraldehyde cross-linked scaffolds. Appling a dual-pump electrospining machine a blend PCL-Gelatin from one side and collagen nanofibers or collagen nanoparticles from the other side were collected on the collector. The fabricated scaffolds were characterized by scanning electron microscopy, contact angle, and mechanical analysis. The cell viability, adhesion and morphology were studied respectively using MTT assay, hoechst staining and scanning electron microscopy. The results indicated significantly improvement of cell viability, adhesion and better spreading on scaffolds with collagen nanoparticles than collagen nanofibers. It seems changes in surface morphology, viscoelastic moduli and swelling ability following cross-linking with glutaraldehyde in scaffold with collagen nanoparticles are still favorable for cellular proliferation. Based on these results, in the case of glutaraldehyde cross-linking, application of collagen nanoparticles rather than collagen nanofibers in tissue regeneration scaffolds will better mimic the extracellular matrix characteristics; and preserve the viability and adhesion of seeded cells.
由于胶原蛋白天然是一种主要的细胞外基质蛋白，它已被广泛应用于皮肤组织工程支架中，以模拟细胞外基质的特性，促进活细胞的适当移植。然而，应用这种天然聚合物存在一些挑战，例如在水性环境中溶解度高，这需要进一步考虑，如化学交联以实现稳定化。但这些处理也会影响其功能，最终影响支架上的细胞行为。在本研究中，我们评估了胶原纳米纤维与胶原纳米颗粒在戊二醛交联支架上对细胞黏附及活力的适用性。使用双泵电纺机，从一侧收集聚己内酯 - 明胶共混物，从另一侧收集胶原纳米纤维或胶原纳米颗粒，并收集在收集器上。通过扫描电子显微镜、接触角和力学分析对制备的支架进行表征。分别使用MTT法、Hoechst染色和扫描电子显微镜研究细胞活力、黏附及形态。结果表明，与胶原纳米纤维相比，含有胶原纳米颗粒的支架上细胞活力显著提高，黏附性更好且铺展更佳。似乎在含有胶原纳米颗粒的支架中，与戊二醛交联后表面形态、粘弹性模量和溶胀能力的变化仍然有利于细胞增殖。基于这些结果，在戊二醛交联的情况下，在组织再生支架中应用胶原纳米颗粒而非胶原纳米纤维将更好地模拟细胞外基质特性，并保持接种细胞的活力和黏附性。
