Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
J Biomater Appl. 2021 Feb;35(7):743-753. doi: 10.1177/0885328220949367. Epub 2020 Aug 17.
In this study, turmeric's active ingredient (Curcumin) was encapsulated into RGD modified Liposomes (RGD-Lip-Cur) its cytotoxic effect on the breast cancer cell line (MCF-7) was evaluated by MTT, flow cytometry and Caspase assay. Liposomes were characterized using transmission electron microscopy (TEM). Results demonstrated that the liposomes were spherical in shape, ranging from 70 to 100 nm. MTT assay revealed that RGD-Lip-Cur had a significant cytotoxic effect on MCF-7 cells at concentrations of 32, 16 and 4 μg/ml compared to Lip-Cur (P < 0.05) and curcumin (P < 0.01). The apoptosis assay demonstrated that RGD-Lip-Cur induces the apoptosis in MCF-7 cells (39.6% vs 40.2% for initial and secondary apoptosis) significantly more than Lip-Cur (67.7% vs 9.16% for initial and secondary apoptosis) and free curcumin (7.84% vs 38.8% for initial and secondary apoptosis). Moreover, caspase assay showed that RGD-Lip-Cur activates caspase 3/7 compared to Lip-Cur (P < 0.05) and free curcumin (P < 0.01). The RGD-Lip-Cur was similar to the control group and had no significant cytotoxicity effect. It is concluded that RGD-Lip-Cur as a novel carrier have high cytotoxicity effect on breast cancer cell line (MCF-7).
在这项研究中，姜黄的活性成分（姜黄素）被包裹在 RGD 修饰的脂质体（RGD-Lip-Cur）中，通过 MTT、流式细胞术和 Caspase 测定评估其对乳腺癌细胞系（MCF-7）的细胞毒性作用。用透射电子显微镜（TEM）对脂质体进行了表征。结果表明，脂质体呈球形，粒径在 70 至 100nm 之间。MTT 测定结果表明，与 Lip-Cur（P<0.05）和姜黄素（P<0.01）相比，RGD-Lip-Cur 在浓度为 32、16 和 4μg/ml 时对 MCF-7 细胞具有显著的细胞毒性作用。凋亡测定表明，RGD-Lip-Cur 诱导 MCF-7 细胞凋亡（初始和次级凋亡分别为 39.6%和 40.2%）明显高于 Lip-Cur（初始和次级凋亡分别为 67.7%和 9.16%）和游离姜黄素（初始和次级凋亡分别为 7.84%和 38.8%）。此外，Caspase 测定表明，与 Lip-Cur（P<0.05）和游离姜黄素（P<0.01）相比，RGD-Lip-Cur 激活了 caspase 3/7。RGD-Lip-Cur 与对照组相似，无明显细胞毒性作用。结论：RGD-Lip-Cur 作为一种新型载体对乳腺癌细胞系（MCF-7）具有高细胞毒性作用。
