Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
Naunyn Schmiedebergs Arch Pharmacol. 2024 Apr;397(4):2347-2357. doi: 10.1007/s00210-023-02752-7. Epub 2023 Oct 13.
Nanotechnology and drug co-delivery offer a novel avenue in drug delivery research liposome-based co-delivery of anticancer drugs targeting the apoptosis pathway as a promising new approach to treat cancer. In this study, a co-delivery system of liposomes (arsenic trioxide/curcumin) modified with RGD peptide was designed to aim for enhancing the treatment of prostate cancer cells (PC3 cell line). Liposomal co-loaded curcumin and arsenic trioxide modified by RGD peptide (NLPs-RGD-Cur-ATO) were prepared by thin-layer lipid hydration techniques for the treatment of prostate cancer. The stability of the NLPs-RGD-Cur-ATO was evaluated by particle size analysis through dynamic light scattering (DLS) analysis and transmission electron microscopy (TEM). The percentage of cytotoxicity and apoptotic effect in PC3 cells treated with NLPs-RGD-Cur-ATO were detected by MTT and Annexin V-FITC (fluorescein isothiocyanate)/PI affinity assay, respectively. The particle size of NLPs-RGD-Cur-ATO was approximately 100 nm, with an encapsulation efficiency of about 99.52% and 70.61%, for ATO and Cur, respectively. Besides, NLPs-RGD-Cur-ATO displayed an enhanced anti-proliferative effect, increased the percentage of apoptotic cells 98 ± 1.85% (p < 0.0001), and significantly reduced EGFR gene expression level (p < 0.001) in the cell line tested. These results indicated that our NLPs-RGD-Cur-ATO co-delivery system was a promising strategy for prostate cancer therapy.
纳米技术和药物共递送为药物递送研究提供了一条新途径，基于脂质体的抗癌药物共递送靶向细胞凋亡途径作为治疗癌症的一种有前途的新方法。在这项研究中，设计了一种共递送系统，即通过 RGD 肽修饰的脂质体（三氧化二砷/姜黄素），旨在增强对前列腺癌细胞（PC3 细胞系）的治疗效果。通过薄层层析水化技术制备载姜黄素和 RGD 肽修饰的三氧化二砷共载脂质体（NLPs-RGD-Cur-ATO），用于治疗前列腺癌。通过动态光散射（DLS）分析和透射电子显微镜（TEM）分析粒径分析评估 NLPs-RGD-Cur-ATO 的稳定性。通过 MTT 和 Annexin V-FITC（荧光素异硫氰酸酯）/PI 亲和测定法分别检测 NLPs-RGD-Cur-ATO 处理的 PC3 细胞的细胞毒性百分率和凋亡作用。NLPs-RGD-Cur-ATO 的粒径约为 100nm，ATO 和 Cur 的包封效率分别约为 99.52%和 70.61%。此外，NLPs-RGD-Cur-ATO 表现出增强的抗增殖作用，使凋亡细胞的百分比增加 98±1.85%（p<0.0001），并显著降低了细胞系中 EGFR 基因的表达水平（p<0.001）。这些结果表明，我们的 NLPs-RGD-Cur-ATO 共递药系统是治疗前列腺癌的一种很有前途的策略。
