骨髓来源免疫细胞的分离和制备用于代谢分析。

Isolation and Preparation of Bone Marrow-Derived Immune Cells for Metabolic Analysis.

机构信息

Department of Immunology, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB, Canada.

Department of Pathology, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB, Canada.

出版信息

Methods Mol Biol. 2020;2184:273-280. doi: 10.1007/978-1-0716-0802-9_19.

DOI:10.1007/978-1-0716-0802-9_19

PMID:32808232

Abstract

The isolation of immune cells from the bone marrow is important for obtaining sufficient numbers for downstream analysis. Immune cells derived from the bone marrow may be subjected to metabolic assays for analysis or used to test the effect of infectious agents on immune cells. Here, we describe a process for the isolation of macrophages, dendritic cells, and neutrophils from mice. Using the methods described herein, specific immune cells with purity above 85-90% can be obtained from the bone marrow of mice.

摘要

从骨髓中分离免疫细胞对于获得足够数量的免疫细胞进行下游分析非常重要。从骨髓中获得的免疫细胞可以进行代谢分析，也可以用于测试感染因子对免疫细胞的影响。在这里，我们描述了一种从小鼠骨髓中分离巨噬细胞、树突状细胞和中性粒细胞的方法。使用本文所述的方法，可以从小鼠骨髓中获得纯度高于 85-90%的特定免疫细胞。