小鼠中性粒细胞和单核细胞的流式细胞术鉴定
Flow cytometric identification of murine neutrophils and monocytes.
作者信息
Lagasse E, Weissman I L
机构信息
Department of Pathology and Developmental Biology, Stanford University School of Medicine, CA 94305, USA.
出版信息
J Immunol Methods. 1996 Oct 16;197(1-2):139-50. doi: 10.1016/0022-1759(96)00138-x.
PMID:8890901
Abstract
Flow cytometry and cell sorting have been employed in order to identify and purify murine neutrophils and monocytes. Using a combination of antibodies, we were able to distinguish between these two subsets of myeloid cells. The method described in this paper is simple to perform and can be applied to characterize myeloid cells from blood, spleen, bone marrow and after an induced inflammation.
摘要
为了鉴定和纯化小鼠中性粒细胞和单核细胞，已采用了流式细胞术和细胞分选技术。通过结合使用多种抗体，我们能够区分这两个髓样细胞亚群。本文所述方法操作简单，可用于表征来自血液、脾脏、骨髓以及诱导炎症后的髓样细胞。
Zotero 插件