Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models.

Wound healing is a fundamental physiological process to keep the integrity of the skin; failure of wound healing leads to chronic wounds, which are a common and severe medical problem. MicroRNAs (miRNAs) are gene regulators important for multiple biological functions in the skin, and they play essential roles in different phases of wound repair. Many miRNAs have been found dysregulated in human chronic wounds. Therefore, miRNAs may serve as potential therapeutic targets for wound treatment. In this chapter, we describe a step-by-step protocol about how to evaluate the therapeutic potential of a miRNA in mouse in vivo and human ex vivo wound models. The findings from these preclinical wound models will serve as a basis for further clinical trials.