Dermatology and Venereology Division, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institute, Stockholm, Sweden.
Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institute, Stockholm, Sweden.
Methods Mol Biol. 2021;2193:67-75. doi: 10.1007/978-1-0716-0845-6_7.
Wound healing is a fundamental physiological process to keep the integrity of the skin; failure of wound healing leads to chronic wounds, which are a common and severe medical problem. MicroRNAs (miRNAs) are gene regulators important for multiple biological functions in the skin, and they play essential roles in different phases of wound repair. Many miRNAs have been found dysregulated in human chronic wounds. Therefore, miRNAs may serve as potential therapeutic targets for wound treatment. In this chapter, we describe a step-by-step protocol about how to evaluate the therapeutic potential of a miRNA in mouse in vivo and human ex vivo wound models. The findings from these preclinical wound models will serve as a basis for further clinical trials.
伤口愈合是保持皮肤完整性的基本生理过程；伤口愈合失败会导致慢性伤口，这是一种常见且严重的医学问题。microRNAs（miRNAs）是皮肤中多种生物学功能的重要基因调控因子，它们在伤口修复的不同阶段发挥着重要作用。许多 miRNA 在人类慢性伤口中被发现失调。因此，miRNAs 可能成为治疗伤口的潜在治疗靶点。在本章中，我们描述了一种逐步方案，用于评估 miRNA 在体内和体外人类伤口模型中治疗小鼠的潜力。这些临床前伤口模型的研究结果将为进一步的临床试验提供基础。
