Center for Translational Ocular Immunology, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA.
Department of Ophthalmology, Tufts Medical Center, Tufts University School of Medicine, Boston, MA, USA.
Methods Mol Biol. 2021;2193:159-174. doi: 10.1007/978-1-0716-0845-6_16.
Plasmacytoid dendritic cells (pDCs) are crucial for corneal homeostasis through secretion of various anti-angiogenic molecules and growth factors. Due to its avascular nature, only a limited number of adoptively transferred cells home to the cornea, when administered systemically. In addition, local adoptive transfer of cells poses several challenges and the clinical application of commonly used techniques is limited. Herein, we detail a novel approach for local adoptive transfer of pDCs to the cornea for the treatment of corneal wounds. This approach utilizes a commonly used fibrin sealant as a means of transferring previously isolated cells locally on the cornea. The technique is simple, reproducible, and is accompanied with successful transfer and integration of a substantial number of the cells to the cornea. Application of this approach to transfer pDCs promotes corneal wound healing. Furthermore, this technique can be applied for adoptive transfer of any cell of interest to the cornea.
浆细胞样树突状细胞（pDCs）通过分泌各种抗血管生成分子和生长因子，对角膜稳态至关重要。由于角膜无血管，当全身给药时，只有有限数量的过继转移细胞归巢到角膜。此外，局部过继转移细胞存在若干挑战，并且常用技术的临床应用受到限制。在此，我们详细介绍了一种将 pDC 局部过继转移到角膜以治疗角膜伤口的新方法。该方法利用一种常用的纤维蛋白密封剂作为将先前分离的细胞局部转移到角膜上的手段。该技术简单、可重现，并且伴随着大量细胞成功转移和整合到角膜中。该方法在转移 pDCs 以促进角膜伤口愈合方面的应用。此外，该技术可用于将任何感兴趣的细胞过继转移到角膜。
