小鼠角膜修复模型：上皮/机械性损伤与基质/化学性损伤。
Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries.
机构信息
Department of Ophthalmology, School of Medicine, University of California, San Francisco, CA, USA.
Francis I. Proctor Foundation, University of California, San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2021;2193:149-158. doi: 10.1007/978-1-0716-0845-6_15.
Abstract
The tissue response to injury is a complex process. The cornea is an excellent model for studying wound repair processes because of its simple anatomy, easy accessibility, and normal avascular state. Here, we describe two corneal repair models in mice: an epithelial/mechanical injury model and a stromal/chemical injury model. The two models induce different repair responses, and consequently enable the study of independent repair processes. Here, we describe how these two wound models may be used to study basic cellular and molecular mechanisms of corneal repair.
摘要
组织对损伤的反应是一个复杂的过程。由于角膜解剖结构简单、易于接近且正常情况下无血管，因此它是研究伤口修复过程的极佳模型。在这里，我们描述了两种在小鼠中进行角膜修复的模型：上皮/机械损伤模型和基质/化学损伤模型。这两种模型可引起不同的修复反应，从而能够研究独立的修复过程。在这里，我们将介绍如何使用这两种伤口模型来研究角膜修复的基本细胞和分子机制。
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