State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Food Funct. 2020 Sep 23;11(9):7468-7480. doi: 10.1039/d0fo01266a.
Oxidative stress, inflammation and gut microbiota disorders can be induced by long-term high-fat diets (HFD). In order to confirm that polyphenols can improve these symptoms, polyphenols from Shanxi-aged vinegar (SAVEP) were extracted, and the components were detected by Comprehensive two-dimensional gas chromatography mass spectrometry (GC × GC-MS). 41 polyphenols include 18 phenolic acids and 17 polyphenols, which have not been reported. The mechanism of SAVEP on oxidative stress and inflammatory stress induced by HFD in rats and its regulating effect on intestinal flora disorder were studied. The results showed that SAVEP could significantly improve the lipid, inflammatory stress and oxidative stress related indicators compared with the Model group ("Model" refers to the group that successfully constructed a hyperlipidemia model by feeding HFD without any drugs or SAVEP in subsequent experiments.). In addition, SAVEP decreased the Firmicutes/Bacteroidetes ratio compared with the Model group, and elevated the relative abundance of beneficial bacteria. Conclusively, SAVEP can alleviate the oxidative stress and inflammatory stress caused by HFD, improving intestinal microbial disorders. The Spearman's correlation analysis revealed that Desulfovibrio, Lactobacillus and Akkermansia were correlated negatively with all of the inflammatory indicators, whereas Ruminococcus was the opposite. These results suggest that SAVEP may be a novel strategy against oxidative stress and inflammation, restoring the normal microbial community ecology of the gut and the treatment of metabolic syndromes.
长期高脂肪饮食（HFD）可诱导氧化应激、炎症和肠道微生物群紊乱。为了证实多酚可以改善这些症状，从山西老陈醋（SAVEP）中提取多酚，并通过全二维气相色谱-质谱联用（GC×GC-MS）检测其成分。41 种多酚包括 18 种酚酸和 17 种多酚，这些成分以前没有报道过。研究了 SAVEP 对 HFD 诱导大鼠氧化应激和炎症应激的作用机制及其对肠道菌群紊乱的调节作用。结果表明，与模型组相比，SAVEP 能显著改善血脂、炎症应激和氧化应激相关指标（“模型”是指通过喂食 HFD 成功构建高脂血症模型但在后续实验中不使用任何药物或 SAVEP 的组）。此外，与模型组相比，SAVEP 降低了厚壁菌门/拟杆菌门的比例，并提高了有益菌的相对丰度。总之，SAVEP 可减轻 HFD 引起的氧化应激和炎症应激，改善肠道微生物失调。Spearman 相关分析显示，脱硫弧菌、乳杆菌和阿克曼氏菌与所有炎症指标呈负相关，而真杆菌则相反。这些结果表明，SAVEP 可能是一种对抗氧化应激和炎症的新策略，可恢复肠道正常微生物群落生态和治疗代谢综合征。
