Core Research Program 1515, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China.
The State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China.
Molecules. 2018 Dec 5;23(12):3213. doi: 10.3390/molecules23123213.
Polyphenols from the L. berry have shown protective effects on experimental non-alcoholic fatty liver disease (NAFLD) in our previous studies. As endotoxins from gut bacteria are considered to be the major trigger of inflammation in NAFLD, this study aims to clarify the regulatory effects of L. berry polyphenols (LCBP) on gut microbiota in a high fat diet (HFD)-induced mouse model. C57BL/6N mice were fed with a normal diet, HFD, or HFD containing 0.5⁻1% of LCBP for 45 days. The results revealed that supplementation with LCBP decreased significantly the levels of IL-2, IL-6, MCP-1, and TNF-α in serum, as well as endotoxin levels in both serum and liver in HFD-fed mice. Fecal microbiota characterization by high throughput 16S rRNA gene sequencing revealed that a HFD increased the / ratio, and LCBP reduced this ratio by increasing the relative abundance of , and another two undefined bacterial genera belonging to the order of and family of , and also by decreasing the relative abundance of six bacterial genera belonging to the phylum , including , , , and . These data demonstrated that LCBP potentially attenuated inflammation in NAFLD through modulation of gut microbiota, especially the ratio of to .
在我们之前的研究中，蓝莓中的多酚对实验性非酒精性脂肪性肝病 (NAFLD) 具有保护作用。由于肠道细菌内毒素被认为是 NAFLD 炎症的主要触发因素，因此本研究旨在阐明蓝莓多酚（LCBP）对高脂肪饮食（HFD）诱导的小鼠模型中肠道微生物群的调节作用。C57BL/6N 小鼠用正常饮食、HFD 或 HFD 喂养 45 天，其中含有 0.5-1%的 LCBP。结果表明，LCBP 补充可显著降低 HFD 喂养小鼠血清中 IL-2、IL-6、MCP-1 和 TNF-α 的水平，以及血清和肝脏中的内毒素水平。通过高通量 16S rRNA 基因测序对粪便微生物群进行表征，结果表明 HFD 增加了/比值，而 LCBP 通过增加属于目和科的两个未定义细菌属的相对丰度来降低该比值，和 ，以及通过降低六个属于门的细菌属的相对丰度来降低该比值，包括，，，和。这些数据表明，LCBP 通过调节肠道微生物群，特别是/比值，可能减轻了 NAFLD 中的炎症。
