Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, Zhejiang Province, China.
Food Funct. 2020 Sep 23;11(9):7830-7841. doi: 10.1039/d0fo00837k.
Steroid-induced avascular necrosis of the femoral head (SANFH) is a major complication of long-term or excessive clinical use of glucocorticoids. Allicin is a classical ingredient extracted from garlic and has many functions such as anti-apoptosis and antibacterial effects. The purpose of this study was to investigate the effect and the mechanism of allicin on apoptosis of osteoblasts induced by dexamethasone (Dex) and SANFH in rats. In vitro, we performed CCK-8, western blotting, TUNEL and other experiments, and the results of these experiments showed that allicin could inhibit the Dex-induced abnormal expression of C-caspase3, C-caspase9, Bax, cytochrome C and Bcl-2 by activating the PI3K/AKT pathway. In vivo, the results of micro-CT, hematoxylin-eosin staining and immunohistochemical analysis suggested that allicin could effectively inhibit the progress of SANFH in rats. In summary, our experiments indicate that allicin is a potential drug for the treatment of SANFH.
激素诱导性股骨头坏死（SANFH）是长期或过量临床应用糖皮质激素的主要并发症。大蒜素是从大蒜中提取的一种经典成分，具有抗细胞凋亡和抗菌等多种功能。本研究旨在探讨大蒜素对地塞米松（Dex）诱导的大鼠成骨细胞凋亡及 SANFH 的作用及机制。体外实验进行了 CCK-8、western blot、TUNEL 等实验，结果表明大蒜素可通过激活 PI3K/AKT 通路抑制 Dex 诱导的 C-caspase3、C-caspase9、Bax、细胞色素 C 和 Bcl-2 的异常表达。体内实验结果显示，micro-CT、苏木精-伊红染色和免疫组织化学分析表明，大蒜素可有效抑制大鼠 SANFH 的进展。综上所述，我们的实验表明大蒜素是治疗 SANFH 的一种潜在药物。
