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环黄芪醇对激素诱导的股骨头坏死有保护作用。

Cyasterone has a protective effect on steroid-induced Osteonecrosis of the femoral head.

机构信息

Department of Sports Medicine, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong Province, China.

Lingnan Medical Research Center of Guangzhou University of Chinese Medicine, Guangzhou, 510405, Guangdong Province, China.

出版信息

PLoS One. 2023 Oct 30;18(10):e0293530. doi: 10.1371/journal.pone.0293530. eCollection 2023.

DOI:10.1371/journal.pone.0293530
PMID:37903142
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10615314/
Abstract

CONTEXT

Cyasterone alleviated the apoptosis of BMSCs induced by Dexamethasone via the PI3K/AKT signaling pathway. In addition, Cyasterone had a protective effect on SIONFH model rats by reducing the percentage of empty bone lacunae.

OBJECTIVE

To study the effect of Cyasterone on apoptosis of rat BMSCs and its function on the SIONFH rat model.

METHODS

Rat BMSCs were cultured and divided into Control, DXM and Cyasterone (DXM+Cyasterone) groups. The apoptosis of each group was detected by flow cytometry, the expressions of Caspase-3 and Caspase-9 were detected by immunofluorescence staining, and the mRNA and protein expressions of AKT, BAX, P53, P85, Bcl-2 and Cytochrome C were detected by qPCR and WB. In animal experiments, the femoral head of rats were subjected to HE staining and Micro-CT to observe the necrosis and repair conditions.

RESULTS

The apoptosis rate of DXM and Cyasterone groups increased compared with Control group, and the apoptosis rate of Cyasterone group decreased compared with DXM group. Compared with DXM group, the mRNA expression of BAX, P53, P85 and Cytochrome C in Cyasterone group were increased, while the protein expression of AKT and Bcl-2 decreased. The histopathological and morphological analysis showed that Cyasterone promoted the trabecular bone structure in rat, which evenly benefit for the repair of SIONFH.

CONCLUSION

Cyasterone can reduce the apoptosis of rat BMSCs induced by Dexamethasone, and help promoting the bone repair in SIONFH rats.

摘要

背景

苍术酮通过 PI3K/AKT 信号通路减轻地塞米松诱导的骨髓间充质干细胞凋亡。此外，苍术酮通过减少空骨陷窝百分比对 SIONFH 模型大鼠具有保护作用。

目的

研究苍术酮对大鼠骨髓间充质干细胞凋亡的影响及其对 SIONFH 大鼠模型的作用。

方法

培养大鼠骨髓间充质干细胞并分为对照组、地塞米松组和苍术酮组（地塞米松+苍术酮）。通过流式细胞术检测各组细胞凋亡情况，免疫荧光染色检测 Caspase-3 和 Caspase-9 的表达，qPCR 和 WB 检测 AKT、BAX、P53、P85、Bcl-2 和细胞色素 C 的 mRNA 和蛋白表达。在动物实验中，对大鼠股骨头进行 HE 染色和 Micro-CT 观察坏死和修复情况。

结果

与对照组相比，地塞米松组和苍术酮组的细胞凋亡率增加，而苍术酮组的细胞凋亡率低于地塞米松组。与地塞米松组相比，苍术酮组的 BAX、P53、P85 和细胞色素 C 的 mRNA 表达增加，而 AKT 和 Bcl-2 的蛋白表达减少。组织病理学和形态学分析表明，苍术酮促进了大鼠的小梁骨结构，均匀地有利于 SIONFH 的修复。

结论

苍术酮可减少地塞米松诱导的大鼠骨髓间充质干细胞凋亡，并有助于促进 SIONFH 大鼠的骨修复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/06e04adc5824/pone.0293530.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/26e9df64a267/pone.0293530.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/0bcfcfca4501/pone.0293530.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/272e1c165aff/pone.0293530.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/8ca3460fb204/pone.0293530.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/d90514cba622/pone.0293530.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/06e04adc5824/pone.0293530.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/26e9df64a267/pone.0293530.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/0bcfcfca4501/pone.0293530.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/272e1c165aff/pone.0293530.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/8ca3460fb204/pone.0293530.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/d90514cba622/pone.0293530.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f0e/10615314/06e04adc5824/pone.0293530.g006.jpg

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