Sangiah S, Caldwell D F
Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078.
Life Sci. 1988;42(15):1425-9. doi: 10.1016/0024-3205(88)90052-5.
Administration of a high dose of glucose (2.5 g/kg, i.p.) that is known to produce severe hyperglycemia in euglycemic rats suppressed rapid eye movement (REM) sleep time significantly during the first three hours of 8 hr total electroencephalogram (EEG) recording period. Co-administration of glucose (2.5 g/kg, i.p.) and a non-convulsive dose of insulin (1.0 I.U./kg, i.p.) produced a significant reduction in REM sleep time during 1st through 5th hour and an increase in slow-wave sleep (NREM) time in the 3rd and 4th hour of 8 hr total EEG recording period. However, awake, NREM and REM sleep time in the 8 hr total EEG recording period were unaffected by either glucose alone or glucose plus insulin treatments. These results strongly suggest that the insulin's effects on the sleep-awake cycle i.e. reduction in REM and a slight increase in NREM sleep times of rats is not due to indirect effects of insulin on the central nervous system via hypoglycemia as reported by us previously, but could possibly be due to its direct effects on brain chemistry of neurotransmitters such as serotonin, catecholamines and acetylcholine which are believed to modulate the sleep-awake cycle pattern in rats.
给正常血糖的大鼠注射已知会使其产生严重高血糖的高剂量葡萄糖（2.5克/千克，腹腔注射），在8小时全脑电图（EEG）记录期的前三个小时内，快速眼动（REM）睡眠时间显著减少。在8小时全EEG记录期的第1至5小时，同时注射葡萄糖（2.5克/千克，腹腔注射）和非惊厥剂量的胰岛素（1.0国际单位/千克，腹腔注射）可使REM睡眠时间显著减少，在第3和第4小时慢波睡眠（NREM）时间增加。然而，在8小时全EEG记录期内，清醒、NREM和REM睡眠时间不受单独葡萄糖或葡萄糖加胰岛素治疗的影响。这些结果有力地表明，胰岛素对睡眠-觉醒周期的影响，即大鼠REM睡眠时间减少和NREM睡眠时间略有增加，并非如我们之前报道的那样是胰岛素通过低血糖对中枢神经系统产生的间接作用，而可能是由于其对血清素、儿茶酚胺和乙酰胆碱等神经递质的脑化学物质产生直接作用，这些神经递质被认为可调节大鼠的睡眠-觉醒周期模式。
