Wang Li, Wang Zhujian, Ma Jinmin, Li Qiongfang, Chen Xueli, Chen Yuhong, Sun Xinghuai
Department of Ophthalmology & Vision Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, China.
NHC Key Laboratory of Myopia, Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China.
Ocul Immunol Inflamm. 2022 Jan 2;30(1):153-159. doi: 10.1080/09273948.2020.1776883. Epub 2020 Aug 18.
To identify viral pathogens in patients with secondary glaucoma associated with anterior segment uveitis and compare metagenomic deep sequencing (MDS) with enzyme-linked immunosorbent assay (ELISA) combined with Witmer-Desmonts coefficient (WDC) evaluation and real-time quantitative polymerase chain reaction (qPCR) on investigating pathogens in aqueous humor.
Aqueous humor from 31 patients, including 22 Posner-Schlossman Syndrome and 9 other anterior uveitis, was assessed pathogens by ELISA combined with WDC evaluation, virus deoxyribonucleic acid (DNA) detection by real-time qPCR and MDS.
Viral pathogens (HCMV or VZV or RV) were detected in 19 out of 31 eyes (61.3%) by real-time qPCR or WDC evaluation. MDS revealed the presence of HCMV DNA sequences in three PSS patients.
Virus is an important pathogen in secondary glaucoma associated with anterior segment uveitis. MDS is a potential etiologic diagnosis tool to seek intraocular viral pathogens for secondary glaucoma associated anterior segment uveitis.
鉴定与前段葡萄膜炎相关的继发性青光眼患者的病毒病原体，并比较宏基因组深度测序（MDS）与酶联免疫吸附测定（ELISA）联合威特默 - 德斯蒙特斯系数（WDC）评估以及实时定量聚合酶链反应（qPCR）在房水病原体检测中的应用。
收集31例患者的房水，其中包括22例波斯纳 - 施洛斯曼综合征患者和9例其他前段葡萄膜炎患者，通过ELISA联合WDC评估、实时qPCR检测病毒脱氧核糖核酸（DNA）以及MDS来评估病原体。
通过实时qPCR或WDC评估，在31只眼中的19只（61.3%）检测到病毒病原体（人巨细胞病毒或水痘 - 带状疱疹病毒或风疹病毒）。MDS在3例波斯纳 - 施洛斯曼综合征患者中发现了人巨细胞病毒DNA序列。
病毒是与前段葡萄膜炎相关的继发性青光眼的重要病原体。MDS是一种潜在的病因诊断工具，可用于寻找与前段葡萄膜炎相关的继发性青光眼的眼内病毒病原体。
