Yu Jinxia, Gao Yane, Bi Hongsheng, Zhang Youhua, Tang Kai, Guo Dadong, Xie Xiaofeng
Department of Ophthalmology, Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, 250002, China.
Department of Ophthalmology, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, 550000, China.
J Ophthalmic Inflamm Infect. 2024 Dec 31;14(1):70. doi: 10.1186/s12348-024-00449-3.
To evaluate the advantages and clinical utility of metagenomic sequencing (MGS) in diagnosing infectious uveitis pathogens.
A retrospective study was conducted on 20 infectious uveitis patients (20 eyes) who received treatments at the Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine from May 2020 to February 2021. Anterior aqueous humor of the patients was collected and analyzed using MGS. Then, pathogenic microorganisms that cause uveitis were identified through bioinformatic analyses based on the sequencing data of MGS. Finally, the pathogens identified by MGS were verified using both enzyme-linked immune sorbent assay (ELISA) and quantitative PCR (qPCR).
MGS was used to detect viral pathogens in four patients, bacterial pathogens in two patients, and viral and bacterial pathogens in one patient. Among these seven subjects, five were verified by either ELISA or qPCR.
MGS holds significant value and promising potential in diagnosing infectious uveitis pathogens. However, it cannot completely replace the traditional diagnostic techniques and still needs to be integrated with conventional methods to enhance the sensitivity and specificity of pathogen detection. As a pioneering technology, MGS will advance the field of pathogen diagnosis in infectious uveitis.
评估宏基因组测序（MGS）在诊断感染性葡萄膜炎病原体方面的优势和临床实用性。
对2020年5月至2021年2月在山东中医药大学附属眼科医院接受治疗的20例感染性葡萄膜炎患者（20只眼）进行回顾性研究。收集患者的前房水并使用MGS进行分析。然后，基于MGS的测序数据通过生物信息学分析鉴定引起葡萄膜炎的致病微生物。最后，使用酶联免疫吸附测定（ELISA）和定量聚合酶链反应（qPCR）对MGS鉴定出的病原体进行验证。
MGS检测出4例患者为病毒病原体，2例患者为细菌病原体，1例患者为病毒和细菌病原体。在这7名受试者中，5名通过ELISA或qPCR得到验证。
MGS在诊断感染性葡萄膜炎病原体方面具有重要价值和广阔前景。然而，它不能完全取代传统诊断技术，仍需与传统方法相结合以提高病原体检测的敏感性和特异性。作为一项开创性技术，MGS将推动感染性葡萄膜炎病原体诊断领域的发展。
