Analysis of enamel structure and mineral density after different bleaching protocols using micro-computed tomography.

OBJECTIVE

This study aims to investigate the effect of three different bleaching applications on structural integrity, mineral volume (MV) and density of the enamel by using micro-computed tomography (Micro-CT) and evaluate the colour effectiveness using CIEDE2000.

MATERIALS AND METHODS

Twenty-four maxillary premolar teeth were divided into three groups ( = 8) (group 1: 40% HP gel with erbium, chromium: yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser activation; group 2: 40% HP gel with diode laser activation; group 3: 16% CP gel). Bleaching protocols were applied and colour alteration was obtained. Data were calculated with CIEDE2000 before and after bleaching. A Micro-CT was used to scan the specimens before and after the bleaching application. Statistical analysis was performed by ANOVA.

RESULTS

No significant difference was observed between the groups for colour changes ( > .05). Micro-CT analysis showed significant differences in structural thickness, structural separation, mineral density and MV for the different ROIs before and after bleaching for all groups (<.05).

CONCLUSIONS

All of the bleaching methods represented similar efficiency. However, bleaching with Er,Cr:YSGG laser was less harmful to enamel in comparison with other bleaching methods. The present results may be useful for establishing a numerical standard for the change in bleaching with laser systems in dental hard tissues.