Institute of Microbial Pharmaceuticals, College of Life and Health Sciences, Northeastern University, Shenyang, Liaoning 110819, PR China.
Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, 2# North Cuihu Road, Kunming, Yunnan 650091, PR China.
Int J Syst Evol Microbiol. 2020 Sep;70(9):5115-5122. doi: 10.1099/ijsem.0.004389. Epub 2020 Aug 18.
A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1, was isolated from the faeces of . Strain YIM 102482-1 grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl. Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1 was a member of the genus and most closely related to NBRC 15706 (97.6 %), NBRC 103089 (97.6 %), KCTC 13959 (96.4 %) and DSM 13485 (96.0 %), respectively. Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1 formed a distinct branch with all type strains of the genus . The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C, iso-C and C, respectively. The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan. The dominant polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipid. The DNA G+C content of YIM 102482-1 was 63.0 mol%. Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 102482-1(=DSM 102156=CCTCC AB 2016023).
一株新型革兰氏阳性、需氧、非孢子形成、不规则短杆状放线菌菌株，被命名为 YIM 102482-1，从. 的粪便中分离得到。菌株 YIM 102482-1 在 30-37°C、pH8.0 和 1.0-3.0%（w/v）NaCl 存在下生长最佳。同样，基于 16S rRNA 基因序列的分析表明，菌株 YIM 102482-1 是 属的成员，与 NBRC 15706（97.6%）、 NBRC 103089（97.6%）、 KCTC 13959（96.4%）和 DSM 13485（96.0%）最为密切相关。此外，基于 16S rRNA 基因序列和基因组序列构建的系统发育树表明，菌株 YIM 102482-1 与 属的所有模式菌株形成了一个独特的分支。主要的全细胞糖和细胞脂肪酸（>10.0%）为核糖和鼠李糖，以及 anteiso-C、iso-C 和 C。主要的menaquinone 是 MK-9，细胞壁肽聚糖中的诊断二氨基氨基酸是 2,4-二氨基丁酸和鸟氨酸。主要的极性脂质由双磷脂酰甘油、磷脂酰甘油和未鉴定的糖脂组成。YIM 102482-1 的 DNA G+C 含量为 63.0 mol%。基于生理、生化和化学分类学数据的分析结果，菌株 YIM 102482-1 代表了 属的一个新种，建议将其命名为. sp. nov.。模式菌株为 YIM 102482-1（=DSM 102156=CCTCC AB 2016023）。
