Guo Ang, Burleigh Robert J, Smith Natasha, Brouard Mark, Burt Michael
The Chemistry Research Laboratory, Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford OX1 3TA, United Kingdom.
J Am Soc Mass Spectrom. 2020 Sep 2;31(9):1903-1909. doi: 10.1021/jasms.0c00167. Epub 2020 Aug 18.
A time-dependent postextraction differential acceleration (PEDA) potential was used to temporally focus increasingly heavy ions in a stigmatic imaging mass spectrometer, allowing them to be imaged with high mass and spatial resolutions over a broad mass-to-charge (/) range. By applying a linearly rising potential to the ion extraction electrode, sequential / ratios were subjected to a changing electric field, allowing their foci to coincide at the detector. Using this approach, at least 75% of the maximum mass resolution was obtained over a 300-600 Da range when the ion microscope was focused around 450 Da, representing more than a 10-fold increase over the conventional single-field PEDA method.
在像散成像质谱仪中，利用随时间变化的提取后微分加速（PEDA）电势对越来越重的离子进行时间聚焦，从而能够在宽质荷比（m/z）范围内以高质量分辨率和空间分辨率对它们进行成像。通过向离子提取电极施加线性上升的电势，连续的m/z比会受到变化的电场作用，使它们的焦点在探测器处重合。采用这种方法，当离子显微镜聚焦在约450 Da时，在300 - 600 Da范围内可获得至少75%的最大质量分辨率，比传统的单场PEDA方法提高了10倍以上。
