Drenckhahn D, Gröschel-Stewart U, Unsicker K
Cell Tissue Res. 1977 Sep 26;183(2):273-9. doi: 10.1007/BF00226624.
Actin and myosin were localized in various salivary glands (parotid, submandibular, sublingual, lingual and Harderian gland) and the exocrine pancrease of rats by indirect immunofluorescence microscopy using specific rabbit antibodies against chicken gizzard myosin and actin. A bright immunofluorescent staining with both antibodies were observed at three main sites: (1) In myoepithelial cells of all salivary glands, (2) in secretory gland cells underneath the cell membrane bordering the acinar lumen (except Harderian and mucous lingual gland), and (3) in epithelial cells of the various secretory ducts (of all glands) in similar distribution as in acinar cells. The present immunohistochemical findings in acinar cells could lend further support to a concept suggesting that myosin and actin are involved in the process of transport and exocytosis of secretory granules.
利用针对鸡胃肌球蛋白和肌动蛋白的特异性兔抗体，通过间接免疫荧光显微镜技术，在大鼠的各种唾液腺（腮腺、颌下腺、舌下腺、舌腺和哈德氏腺）以及外分泌胰腺中定位肌动蛋白和肌球蛋白。用这两种抗体均观察到在三个主要部位有明亮的免疫荧光染色：（1）在所有唾液腺的肌上皮细胞中；（2）在腺泡腔相邻细胞膜下方的分泌腺细胞中（哈德氏腺和舌黏液腺除外）；（3）在各种分泌导管（所有腺体的）上皮细胞中，其分布与腺泡细胞相似。腺泡细胞中目前的免疫组织化学研究结果可能进一步支持这样一种观点，即肌球蛋白和肌动蛋白参与分泌颗粒的运输和胞吐过程。
