Cao Jia-Qian, Jin Peng-Fei, Li Jing-Xin, Zeng Zhao-Zhun, Zhang Li, Meng Fan-Yue, Chu Kai, Wang Yuan-Yuan, Zhu Feng-Cai
School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.
Vaccine Clinical Evaluation Department, Jiangsu Province Center for Disease Control and Prevention, Nanjing, Jiangsu, People's Republic of China.
Open Forum Infect Dis. 2020 May 26;7(8):ofaa181. doi: 10.1093/ofid/ofaa181. eCollection 2020 Aug.
In this study, we investigated the effects of prior influenza exposure on vaccine-elicited humor immune responses to circulating influenza variants.
We randomly selected 360 participants in previous clinical trials stratified by age. Blood samples were collected and tested by hemagglutination-inhibition tests during the 2015-2016 influenza seasons in China. The antigenic map was plotted and antigenic distance was calculated.
Subjects with H1-priming had higher cross-reactive antibodies titers against A/JiangsuTinghu/11019/2015(H3N2) compared with subjects with B-priming did ( = .038). Subjects with H1-priming also had higher cross-reactive antibodies titers against A/Jiangsu Qinhuai/11059/2015(H3N2) than subjects with both H1 and B priming ( = .036). Nevertheless, subjects with no H1 and B-priming had higher cross-reactive antibodies titers against A/Jiangsu Qinhuai/11059/2015(H3N2) than subjects with both H1 and B priming ( = .012). Antigenic distance was well matched with serological results. Moeover, age-specific differences in human postvaccination responses against the identical circulating strain was noted. In addition, children had the most cross-reactive response to both H3N2 and B-yamagata subtypes.
Our results suggest that prior exposure to H1 or B influenza virus may influence cross-reactivity of H3-specific postvaccination responses and consequently could influence the vaccine effectiveness. Our findings also support that there are age-specific differences in human postvaccination responses.
在本研究中，我们调查了既往流感暴露对疫苗引发的针对流行流感变体的体液免疫反应的影响。
我们从既往临床试验中按年龄分层随机选取360名参与者。在中国2015 - 2016年流感季节期间采集血样并通过血凝抑制试验进行检测。绘制抗原图谱并计算抗原距离。
与B型初免的受试者相比，H1型初免的受试者针对A/江苏亭湖/11019/2015（H3N2）的交叉反应抗体滴度更高（P = 0.038）。H1型初免的受试者针对A/江苏秦淮/11059/2015（H3N2）的交叉反应抗体滴度也高于同时进行H1和B型初免的受试者（P = 0.036）。然而，未进行H1和B型初免的受试者针对A/江苏秦淮/11059/2015（H3N2）的交叉反应抗体滴度高于同时进行H1和B型初免的受试者（P = 0.012）。抗原距离与血清学结果匹配良好。此外，注意到人类针对相同流行毒株的疫苗接种后反应存在年龄特异性差异。另外，儿童对H3N2和B - 山形亚型均具有最强的交叉反应。
我们的结果表明，既往暴露于H1或B型流感病毒可能影响接种疫苗后H3特异性反应的交叉反应性，进而可能影响疫苗效力。我们的研究结果还支持人类疫苗接种后反应存在年龄特异性差异。
