Tertti R, Eerola E, Granfors K, Lahesmaa-Rantala R, Pekkola-Heino K, Toivanen A
Department of Medical Microbiology, Turku University, Finland.
Infect Immun. 1988 May;56(5):1295-300. doi: 10.1128/iai.56.5.1295-1300.1988.
We have determined the opsonic capacity of specific antibodies in patient sera obtained after Yersinia infection. The results indicate that Yersinia antibodies lead to complement activation through the classical pathway, thus overcoming the inhibition of complement-mediated opsonization in the absence of specific antibodies provided by the virulence plasmid in Yersinia enterocolitica and Yersinia pseudotuberculosis. Further, antibodies against plasmid-encoded structures, the Yersinia outer membrane proteins (YOPs), are not necessary in this effect. This is indicated by two facts. (i) Monoclonal antibodies directed against the O polysaccharide of Y. enterocolitica O:3 are capable of opsonizing the plasmid-containing bacteria through C1q binding. (ii) Rabbit antisera show opsonic activity when obtained by immunization both with plasmid-containing Y. enterocolitica expressing the YOPs and a plasmid-cured variant not expressing these proteins.
我们已经测定了耶尔森菌感染后患者血清中特异性抗体的调理吞噬能力。结果表明，耶尔森菌抗体通过经典途径导致补体激活，从而克服了小肠结肠炎耶尔森菌和假结核耶尔森菌毒力质粒所提供的特异性抗体缺失时补体介导的调理吞噬作用的抑制。此外，针对质粒编码结构耶尔森菌外膜蛋白（YOPs）的抗体在这种效应中并非必需。这由两个事实表明。（i）针对小肠结肠炎耶尔森菌O:3 O多糖的单克隆抗体能够通过C1q结合调理含质粒的细菌。（ii）兔抗血清在通过用表达YOPs的含质粒小肠结肠炎耶尔森菌和不表达这些蛋白的质粒缺失变体免疫获得时显示出调理活性。
