Kittelberger R, Hilbink F, Hansen M F, Ross G P, Joyce M A, Fenwick S, Heesemann J, Wolf-Watz H, Nielsen K
Central Animal Health Laboratory, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. kittelbergerr/wallaceville.mqm.govt.nz
Vet Microbiol. 1995 Dec;47(3-4):271-80. doi: 10.1016/0378-1135(95)00121-2.
Yersinia outer protein (YOP) preparations from Y. enterocolitica and Y. pseudotuberculosis were used as antigens in immunoblots for the detection of Yersinia infections in experimentally and naturally infected ruminants. Sera from 9 groups of animals were used: (1) 51 sera from cattle which were false-positive in the standard brucellosis serological tests, (2) 52 sera from brucellosis-negative cattle, (3) 51 sera from a deer herd in which 16 animals were positive in the brucellosis tests and Yersina species were isolated from 5 animals, (4) 50 sera from a deer herd in which sera from all animals were negative in the brucellosis tests, (5) 107 sera from brucellosis-negative cattle which were received from throughout New Zealand, (6) 30 sera from cattle naturally infected with B. abortus and from which B. abortus was isolated, (7) 55 sera from cattle naturally infected with B. abortus, (8) 26 sera from cattle experimentally infected with B. abortus, with mostly high titres in the conventional brucellosis tests, and (9) sera taken weekly from 3 cattle experimentally infected with Y. enterocolitica 0:9. In all 3 Y. enterocolitica 0:9 experimentally infected animals the antibody reactivity against major YOPs in the Y. enterocolitica and in the Y. pseudotuberculosis YOP preparation correlated well with the strength in the classical brucellosis tests and with the staining of smooth lipopolysaccharides (SLPS) in blots, thus confirming the usefulness of YOPs for the detection of Yersinia infections. Sera from naturally infected cattle and deer herds, regardless of whether they were false positive or negative in the brucellosis tests, showed high frequencies of staining in YOP blots (53-58% in cattle and 80-100% in deer), indicating a high prevalence of field infections with Yersinia species in New Zealand. In two of the three sera groups from B. abortus infected animals, antibodies against YOPs were detected with high frequency, showing that dual infections may be common and may interfere with differential serological testing.
将小肠结肠炎耶尔森菌和假结核耶尔森菌的耶尔森菌外膜蛋白（YOP）制剂用作免疫印迹中的抗原，以检测实验性和自然感染反刍动物中的耶尔森菌感染。使用了9组动物的血清：（1）51份来自牛的血清，这些牛在标准布鲁氏菌病血清学检测中呈假阳性；（2）52份来自布鲁氏菌病阴性牛的血清；（3）51份来自鹿群的血清，其中16只动物布鲁氏菌病检测呈阳性，5只动物分离出耶尔森菌属；（4）50份来自鹿群的血清，该鹿群所有动物的血清布鲁氏菌病检测均为阴性；（5）107份来自新西兰各地的布鲁氏菌病阴性牛的血清；（6）30份来自自然感染流产布鲁氏菌且分离出流产布鲁氏菌的牛的血清；（7）55份来自自然感染流产布鲁氏菌的牛的血清；（8）26份来自实验性感染流产布鲁氏菌的牛的血清，在传统布鲁氏菌病检测中大多具有高滴度；（9）每周从3只实验性感染小肠结肠炎耶尔森菌0:9的牛采集的血清。在所有3只实验性感染小肠结肠炎耶尔森菌0:9的动物中，针对小肠结肠炎耶尔森菌和假结核耶尔森菌YOP制剂中主要YOP的抗体反应性与经典布鲁氏菌病检测中的强度以及印迹中光滑脂多糖（SLPS）的染色情况密切相关，从而证实了YOPs在检测耶尔森菌感染方面的有用性。来自自然感染牛群和鹿群的血清，无论它们在布鲁氏菌病检测中是假阳性还是阴性，在YOP印迹中均显示出高频率的染色（牛中为53 - 58%，鹿中为80 - 100%），表明新西兰耶尔森菌属的野外感染患病率很高。在来自流产布鲁氏菌感染动物血清的三个组中的两组中，高频检测到针对YOPs的抗体，表明双重感染可能很常见，并且可能干扰血清学鉴别检测。
