硫酸鱼精蛋白对膀胱表面糖胺聚糖抗菌活性的可逆失活作用。
Reversible inactivation of bladder surface glycosaminoglycan antibacterial activity by protamine sulfate.
作者信息
Parsons C L, Stauffer C W, Schmidt J D
机构信息
Department of Surgery, University of California Medical Center, San Diego 92103.
出版信息
Infect Immun. 1988 May;56(5):1341-3. doi: 10.1128/iai.56.5.1341-1343.1988.
Abstract
Prior studies in our laboratory have shown that the bladder surface is lined with glycosaminoglycans which appear to be an important antibacterial defense mechanism that operates by resisting bacterial adherence and infection. The present study further implicates bladder surface glycosaminoglycans as the key antiadherent factor and also suggests a potential model for diseases (such as urinary tract infections) whereby the antiadherent surface of the bladder is inactivated biochemically. Protamine sulfate treatment of bladder tissue was found to significantly increase bacterial adherence to the urinary bladder by approximately 2.3-fold. This effect was reversed by a second treatment of the bladder with pentosanpolysulfate (a polysaccharide known to duplicate the surface antiadherent effect). Protamine sulfate had no effect on bacterial viability or bacterial adherence when bacteria were pretreated with it.
摘要
我们实验室之前的研究表明，膀胱表面覆盖有糖胺聚糖，这似乎是一种重要的抗菌防御机制，通过抵抗细菌黏附和感染发挥作用。本研究进一步表明膀胱表面糖胺聚糖是关键的抗黏附因子，还提出了一种疾病（如尿路感染）的潜在模型，即膀胱的抗黏附表面通过生化方式失活。发现用硫酸鱼精蛋白处理膀胱组织可使细菌对膀胱的黏附显著增加约2.3倍。用聚戊糖硫酸酯（一种已知可复制表面抗黏附作用的多糖）再次处理膀胱可逆转这种效应。当用硫酸鱼精蛋白预处理细菌时，它对细菌活力或细菌黏附没有影响。
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